Lee Ann Laurent-Applegate

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Cryopreservation and lyophilization processes are widely used for conservation purposes in the pharmaceutical, biotechnological, and food industries or in medical transplantation. Such processes deal with extremely low temperatures (e.g., -196 degrees C) a ...

Articular cartilage regeneration poses particularly tough challenges for implementing cell-‐based therapies. Many cell types have been investigated looking for a balanced combination of responsiveness and stability, yet techniques are still far from defin ...

Current restrictions for human cell-based therapies have been related to technological limitations with regards to cellular proliferation capacity and maintenance of differentiated phenotype for primary human cell culture. In our experience, we have seen t ...

Few biopharmaceutical preparations developed from biologicals are available for tissue regeneration and scar management. When developing biological treatments with cellular therapy, selection of cell types and establishment of consistent cell banks are cru ...

Cell therapy for nucleus pulposus regeneration is an attractive treatment for early disc degeneration as shown by studies using autologous nucleus pulposus cells or stem cells. Another potential source of cells is fetal cells. We investigated the feasibili ...

Engineering of fetal tissue has a high potential for the treatment of acute and chronic wounds of the skin in humans as these cells have high expansion capacity under simple culture conditions and one organ donation can produce Master Cell Banks which can ...

Fetal bone cells were shown to have an interesting potential for therapeutic use in bone tissue engineering due to their rapid growth rate and their ability to differentiate into mature osteoblasts in vitro. We describe hereafter their capability to promot ...

During the last decade, extensive research has been performed in the field of orthopedic medicine to develop cell-based therapies for the restoration of injured bone tissue. We previously demonstrated that human primary fetal bone cells (HFBCs) associated ...

Chronic and acute tendon injuries are frequent afflictions, for which treatment is often long and unsatisfactory. When facing extended injuries, matrices and scaffolds with sufficient biomechanical properties are required for surgical repair and could addi ...

Hand tendon/ligament structural ruptures (tears, lacerations) often require surgical reconstruction and grafting, for the restauration of finger mechanical functions. Clinical-grade human primary progenitor tenocytes (FE002 cryopreserved progenitor cell so ...

We describe herein some immunological properties of human fetal bone cells recently tested for bone tissue engineering applications. Adult mesenchymal stem cells and adult osteoblasts were included in the study for comparison. Surface markers involved in b ...

The aim of this study was to culture human fetal bone cells (dedicated cell banks of fetal bone derived from 14 week gestation femurs) within both hyaluronic acid gel and collagen foam, to compare the biocompatibility of both matrices as potential delivery ...

Different cell sources for bone tissue engineering are reviewed. In particular, adult cell source strategies have been based on the implantation of unfractionated fresh bone marrow; purified, culture expanded mesenchymal stem cells, differentiated osteobla ...

Bioresorbable scaffolds made Of poly(L-lactic acid) (PLA) obtained by supercritical gas foaming were recently described as suitable for tissue engineering, portraying biocompatibility with primary osteoblasts in vitro and interesting mechanical properties ...

Cartilage repair remains a major challenge in human orthopedic medicine, necessitating the application of innovative strategies to overcome existing technical and clinical limitations. Adhesive hydrogels have emerged as promising candidates for cartilage r ...

Emerging 3D printing technology permits innovative approaches to manufacture cartilage scaffolds associated with layer-by-layer mechanical property adaptation. However, information about gradients of mechanical properties in human articular cartilage is li ...

For tissue engineering, several cell types and tissues have been proposed as starting material. Allogenic skin products available for therapeutic usage are mostly developed with cell culture and with foreskin tissue of young individuals. Fetal skin cells o ...

The potential of human fetal bone cells for successful bone regeneration has been shown in vivo. In particular, it has been demonstrated that the seeding of these cells in porous poly-(L-lactic acid)/ ß-tricalcium phosphate scaffolds improved the bone form ...

Multi-drug resistant Pseudomonas aeruginosa has increased progressively and impedes further regression in mortality in burn patients. Such wound infections serve as bacterial reservoir for nosocomial infections and are associated with signi cant morbidity ...

Current restrictions for human cell-based therapies have been related to technological limitations with regards to cellular proliferation capacity (simple culture conditions), maintenance of differentiated phenotype for primary human cell culture and trans ...

Lee Ann Laurent-Applegate

Chattez avec Graph Search

Industrial Biotechnology Conservation Processes: Similarities with Natural Long-Term Preservation of Biological Organisms

Epiphyseal chondro-progenitors provide a stable cell source for cartilage cell therapy

Consistency of fetal cell banks for research and clinical use

Biologicals and fetal cell therapy for wound healing and scar management

Isolation and in vitro chondrogenic potential of human fetal spine cells

Chronic wound healing by fetal cell therapy may be explained by differential gene profiling observed in fetal versus old skin cells

Human fetal bone cells associated with ceramic reinforced PLA scaffolds for tissue engineering

Regulation of proliferation and differentiation of human fetal bone cells

Efficient decellularization of equine tendon with preserved biomechanical properties and cytocompatibility for human tendon surgery indications

Bio-Enhanced Neoligaments Graft Bearing FE002 Primary Progenitor Tenocytes: Allogeneic Tissue Engineering & Surgical Proofs-of-Concept for Hand Ligament Regenerative Medicine

In vitro characterization of immune-related properties of human fetal bone cells for potential tissue engineering applications

Human fetal bone cells in delivery systems for bone engineering

Bone tissue engineering using fetal cell therapy

Repair of critical size defects in the rat cranium using ceramic-reinforced PLA scaffolds obtained by supercritical gas foaming

Cartilage Repair: Promise of Adhesive Orthopedic Hydrogels

Depth-dependent mechanical properties of human articular cartilage obtained by indentation measurements

Wound healing gene-family expression differences between fetal and foreskin cells used for bioengineered skin substitutes

Human bone progenitor cells for clinical application: what kind of immune reaction does fetal xenograft tissue trigger in immuno-competent rats?

Anti-microbial dendrimers against multidrug-resistant P. aeruginosa enhance the angiogenic effect of biological burn-wound bandages

Whole-cell bioprocessing of human fetal cells for tissue engineering of skin