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Pierre Gönczy

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Publications associées (110)

Homogeneous multifocal excitation for high-throughput super-resolution imaging

Pierre Gönczy, Virginie Hamel, Suliana Manley, Paul Guichard, Dora Mahecic, Kyle Michael Douglass, Denis Fortun, Niccolo Banterle, Khalid Ashraf Mohie Ibrahim, Davide Gambarotto

Super-resolution microscopies have become an established tool in biological research. However, imaging throughput remains a main bottleneck in acquiring large datasets required for quantitative biology. Here we describe multifocal flat illumination for fie ...

2020

Novel features of centriole polarity and cartwheel stacking revealed by cryo-tomography

Pierre Gönczy, Davide Demurtas, Alexandra Bezler, Sergey Nazarov, Paul Guichard, Veronika Nemcíková Villímová, Georgios Hatzopoulos

Centrioles are polarized microtubule-based organelles that seed the formation of cilia, and which assemble from a cartwheel containing stacked ring oligomers ofSAS-6 proteins. A cryo-tomography map of centrioles from the termite flagellateTrichonymphaspp. ...

2020

Centriole foci persist in starfish oocytes despite Polo-like kinase 1 inactivation or loss of microtubule nucleation activity

Pierre Gönczy, Marie Juliette Ségolène Pierron, Nils Kalbfuss

Centrioles must be eliminated or inactivated from the oocyte to ensure that only the two functional centrioles contributed by the sperm are present in the zygote. Such removal can occur during oogenesis, as in Drosophila, where departure of Polo kinase fro ...

2020

Multicolor Single-Particle Reconstruction of Protein Complexes

Pierre Gönczy, Suliana Manley, Kyle Michael Douglass, Christian Sieben, Niccolo Banterle

CELL PRESS2019

Live imaging screen reveals that TYRO3 and GAK ensure accurate spindle positioning in human cells

Pierre Gönczy, Coralie Busso, Benita Wolf

Proper spindle positioning is crucial for spatial cell division control. Spindle positioning in human cells relies on a ternary complex comprising G alpha i1-3, LGN and NuMA, which anchors dynein at the cell cortex, thus enabling pulling forces to be exert ...

Springer2019

Aurora A depletion reveals centrosome-independent polarization mechanism in Caenorhabditis elegans

Pierre Gönczy, Coralie Busso, Lukas von Tobel, Christian Gentili, Marie Juliette Ségolène Pierron, Kerstin Klinkert Fabbri, Sarah Herrman

How living systems break symmetry in an organized manner is a fundamental question in biology. In wild-type Caenorhabditis elegans zygotes, symmetry breaking during anterior-posterior axis specification is guided by centrosomes, resulting in anterior-direc ...

2019

Centriole assembly at a glance

Pierre Gönczy, Georgios Hatzopoulos

The centriole organelle consists of microtubules (MTs) that exhibit a striking 9-fold radial symmetry. Centrioles play fundamental roles across eukaryotes, notably in cell signaling, motility and division. In this Cell Science at a Glance article and accom ...

2019

Tissue- and sex-specific small RNAomes reveal sex differences in response to the environment

Laurent Keller, Pierre Gönczy, Frédéric Schütz, Alexandra Bezler, Raffaella Conconi

RNA interference (RNAi) related pathways are essential for germline development and fertility in metazoa and can contribute to inter- and trans-generational inheritance. In the nematode Caenorhabditis elegans, environmental double-stranded RNA provided by ...

2019

High-speed photothermal off-resonance atomic force microscopy reveals assembly routes of centriolar scaffold protein SAS-6

Pierre Gönczy, Santiago Harald Andany, Georg Fantner, Adrian Pascal Nievergelt, Niccolo Banterle

The self-assembly of protein complexes is at the core of many fundamental biological processes1, ranging from the polymerization of cytoskeletal elements, such as microtubules2, to viral capsid formation and organelle assembly3. To reach a comprehensive un ...

2018

Multicolor single-particle reconstruction of protein complexes

Pierre Gönczy, Suliana Manley, Kyle Michael Douglass, Christian Sieben, Niccolo Banterle

Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reco ...

2018

Integrated Microfluidic Device for Drug Studies of Early C. Elegans Embryogenesis

Thomas Lehnert, Martinus Gijs, Pierre Gönczy, Matteo Cornaglia, Li Dong, Radek Jankele

2018

Interaction between the Caenorhabditis elegans centriolar protein SAS-5 and microtubules facilitates organelle assembly

Pierre Gönczy, Nicola Jane Dynes

2018

PI(4,5)P-2 forms dynamic cortical structures and directs actin distribution as well as polarity in Caenorhabditis elegans embryos

Pierre Gönczy, Alessandro De Simone, Melina Jasmin Scholze

2018

Reconstruction From Multiple Particles for 3D Isotropic Resolution in Fluorescence Microscopy

Michaël Unser, Pierre Gönczy, Virginie Hamel, Paul Guichard, Denis Fortun, Niccolo Banterle

Institute of Electrical and Electronics Engineers2018

The Rise of the Cartwheel: Seeding the Centriole Organelle

Pierre Gönczy, Virginie Hamel, Paul Guichard

2018

Uncovering the balance of forces driving microtubule aster migration in C. elegans zygotes

Pierre Gönczy, Coralie Busso, Alessandro De Simone, Antoine Jérôme Spahr

Nature Research2018

ZYG-1 promotes limited centriole amplification in the C. elegans seam lineage

Pierre Gönczy, Fernando Romero Balestra, Benita Wolf, Antoine Jérôme Spahr

2018

TRACMIT: An effective pipeline for tracking and analyzing cells on micropatterns through mitosis

Olivier Burri, Pierre Gönczy, Arne Seitz, Benita Wolf

The use of micropatterns has transformed investigations of dynamic biological processes by enabling the reproducible analysis of live cells using time-lapse fluorescence microscopy. With micropatterns, thousands of individual cells can be efficiently image ...

2017

Zika virus causes supernumerary foci with centriolar proteins and impaired spindle positioning

Pierre Gönczy, Coralie Busso, Benita Wolf

Zika virus (ZIKV) causes congenital microcephaly. Although ZIKV can impair cell cycle progression and provoke apoptosis, which probably contributes to disease aetiology through depletion of neural progenitor cells, additional cellular mechanisms may be imp ...

Royal Society, The2017

Computer simulations reveal mechanisms that organize nuclear dynein forces to separate centrosomes

Pierre Gönczy, Alessandro De Simone

Centrosome separation along the surface of the nucleus at the onset of mitosis is critical for bipolar spindle assembly. Dynein anchored on the nuclear envelope is known to be important for centrosome separation, but it is unclear how nuclear dynein forces ...

Amer Soc Cell Biology2017

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