Publications de Philippe Pelupessy

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Para-water is an analogue of para-hydrogen, where the two proton spins are in a quantum state that is antisymmetric under permutation, also known as singlet state. The populations of the nuclear spin states in para-water are believed to have long lifetimes ...

Understanding the molecular determinants underlying protein function requires the characterization of both structure and dynamics at atomic resolution. Nuclear relaxation rates allow a precise characterization of protein dynamics at the Larmor frequencies ...

Owing to its imidazole side chain, histidine participates in various processes such as enzyme catalysis, pH regulation, metal binding, and phosphorylation. The determination of exchange rates of labile protons for such a system is important for understandi ...

Although labile protons that are exchanging rapidly with those of the solvent cannot be observed directly, their exchange rate constants can be determined by indirect detection of scalar-coupled neighboring nuclei. We have used heteronuclear NMR spectrosco ...

In magnetic resonance imaging (MRI), it is possible to cancel the effects of severe inhomogeneities of the magnetic field even if the field profile is unknown. The new ‘cross-encoded’ method is based on adiabatic frequency-modulated pulses combined with tw ...

New schemes are introduced that allow one to improve the resolution in the indirect dimension of single-scan 'ultrafast' two-dimensional NMR spectra. The methods combine undersampling with band-selective pulses to recover signals that lie outside the detec ...

By monitoring the effect of deuterium decoupling on the decay of transverse N-15 magnetization in D-N-15 spin pairs during multiple-refocusing echo sequences, we have determined fast D-D exchange rates k(D) and compared them with fast H-H exchange rates k( ...

Nuclear magnetic resonance is used to investigate the backbone dynamics in 6-phosphogluconolactonase from Trypanosoma brucei (Tb6PGL) with (holo-) and without (apo-) 6-phosphogluconic acid as ligand. Relaxation data were analyzed using the model-free appro ...

In spatially encoded MRI, the signal is acquired sequentially for different coordinates. In particular for single-scan acquisitions in inhomogeneous fields, spatially encoded methods improve the image quality compared to traditional k-space encoding. Previ ...

Intrinsically disordered proteins and intrinsically disordered regions (IDRs) are ubiquitous in the eukaryotic proteome. The description and understanding of their conformational properties require the development of new experimental, computational, and th ...

We report the in situ and real-time monitoring of the interconversion of L- and D-alanine-d(3) by alanine racemase from Bacillus stearothermophilus directly observed by H-2 NMR spectroscopy in anisotropic phase. The enantiomers are distinguished by the dif ...

The principal components and orientations of the chem. shift anisotropy (CSA) tensors of the carbonyl (C'), nitrogen (N), and amide proton (HN) nuclei of 64 distinct amide bonds in human ubiquitin have been detd. in isotropic soln. by a set of 14 complemen ...

A new method has been developed to obtain dynamic and structural information about peptide planes in proteins by a combination of measurements of weak short-range cross-correlation rates R(HNN/NC') that are due to concerted fluctuations of the HN-N and N-C ...

Philippe Pelupessy

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Challenges in preparing, preserving and detecting para-water in bulk: overcoming proton exchange and other hurdles

Nanosecond Time Scale Motions in Proteins Revealed by High-Resolution NMR Relaxometry

Fast Proton Exchange in Histidine: Measurement of Rate Constants through Indirect Detection by NMR Spectroscopy

Exchange Rate Constants of Invisible Protons in Proteins Determined by NMR Spectroscopy

Cross-encoded magnetic resonance imaging in inhomogeneous fields

Improving resolution in single-scan 2D spectroscopy

Kinetic isotope effects for fast deuterium and proton exchange rates

Insights into internal dynamics of 6-phosphogluconolactonase from Trypanosoma brucei studied by nuclear magnetic resonance and molecular dynamics

The effects of molecular diffusion in spatially encoded magnetic resonance imaging

Distribution of Pico- and Nanosecond Motions in Disordered Proteins from Nuclear Spin Relaxation

On-the-Fly Kinetics of Enzymatic Racemization Using Deuterium NMR in DNA-Based Chiral Oriented Media

Chemical shift anisotropy tensors of carbonyl, nitrogen, and amide proton nuclei in proteins through cross-correlated relaxation in NMR spectroscopy

Anisotropic Local Motions and Location of Amide Protons in Proteins

Determination of Dihedral psi Angles in Large Proteins by Combining NHN/CαHα Dipole/Dipole Cross Correlation and Chemical Shifts

Accurate Measurement of Longitudinal Cross-Relaxation Rates in Nuclear Magnetic Resonance

Protein Backbone Dynamics through 13C’ - 13Cα Cross-Relaxation in NMR Spectoscopy