Publications de Marc Schiltz | EPFL Graph Search

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The X-ray polarization anisotropy of anomalous scattering in crystals of brominated nucleic acids and selenated proteins is shown to have signiﬁcant effects on the diffraction data collected at an absorption edge. For conventionally collected single- or mu ...

Throughout evolution, one of the most ancient forms of aggression between cells or organisms has been the production of proteins or peptides affecting the permeability of the target cell membrane. This class of virulence factors includes the largest family ...

In this paper the anisotropy of anomalous scattering at the Br K-absorption edge in brominated nucleotides is investigated, and it is shown that this effect can give rise to a marked directional dependence of the anomalous signal strength in X-ray diffract ...

Polarized fluorescence spectra were recorded in the vicinity of the Se and Br K edges on crystals of the selenated protein aldose reductase in complex with a brominated inhibitor molecule. The X-ray absorption, refraction and resonant scattering tensors as ...

X-ray induced anisotropic variations of cell parameters in porcine pancreatic elastase (PPE) were used in a multi-Pawley refinement in order to improve the deconvolution of overlapping peaks occurring in the high-angle region of the powder pattern. The ben ...

The preparation of single crystals suitable for X-ray analysis is frequently the most difﬁcult step in structural studies of proteins.With the aid of two examples, it is shown that de novo solution of the crystallographic phase problem can be achieved at l ...

Radiation damage in macromolecular crystals is not suppressed even at 90 K. This is particularly true for covalent bonds involving an anomalous scatterer (such as bromine) at the `peak wavelength'. It is shown that a series of absorption spectra recorded o ...

This work augments the proposal of Schwarzenbach & Flack [J. Appl. Cryst. (1989), 22, 601-605], who have advocated the use of a diffractometer-independent definition of the azimuthal angle

\psi

to specify the diffractiongeometry of a Bragg reflection. It ...

Here we present a biophysical, structural, and computational analysis of the directed evolution of the human DNA repair protein O-6-alkylguanine-DNA alkyltransferase (hAGT) into SNAP-tag, a self-labeling protein tag. Evolution of hAGT led not only to incre ...

Aerolysin is a major virulence factor produced by the Gram-negative bacterium Aeromonas hydrophila and is a member of the beta-pore-forming toxin family. Two oligomerization-deficient aerolysin mutants, H132D and H132N, have been overproduced, proteolyzed ...

Here, the crystallization and initial phasing of the C-terminal domain of human KIN17, a 45 kDa protein mainly expressed in response to ionizing radiation and overexpressed in certain tumour cell lines, are reported. Crystals diffracting to 1.4 Å resolutio ...

Following the seminal work of Von Dreele, high quality powder X-ray diffraction studies on proteins are being established as a valuable complementary technique to single-crystal measurements. Several studies using a variety of experiments approaches have b ...

PTPA, an essential and specific activator of protein phosphatase 2A (PP2A), functions as a peptidyl prolyl isomerase (PPIase). We present here the crystal structures of human PTPA and of the two yeast orthologs (Ypa1 and Ypa2), revealing an all α-helical p ...

Protein powder diffraction is shown to be suitable for obtaining de novo solutions to the phase problem at low resolution via phasing methods such as the isomorphous replacement method. Two heavy-atom derivatives (a gadolinium derivative and a holmium deri ...

A previous analysis of the average intensity and mean-square intensity difference of Friedel opposites, conﬁned to the space group P1 [Flack & Shmueli (2007). Acta Cryst. A63, 257–265], is here extended to all the noncentrosymmetric space groups. The prese ...

Site-specific radiation damage on anomalously scattering sites can be used to generate additional phase information in standard single- or multi-wavelength anomalous diffraction (SAD or MAD) experiments. In this approach the data are kept unmerged, down to ...

We present here the structure of Yer0l0c protein of unknown function, solved by Multiple Anomalous Diffraction and revealing a common fold and oligomerization state with proteins of the regulator of ribonuclease activity A (RraA) family. In Escherichia col ...

Synthesis, properties and reactions of two isomeric dipyrido-1,4-dithiins of the C-2h and C-2v symmetry are described. Their structure detennination and identification are based on spectroscopic methods (H-1 and C-13 NMR, HETCOR, gHMBC and MS), physical pr ...

Marc Schiltz

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Exploiting the anisotropy of anomalous scattering boosts the phasing power of SAD and MAD experiments

Dual Chaperone Role of the C-Terminal Propeptide in Folding and Oligomerization of the Pore-Forming Toxin Aerolysin

Polarization-dependence of anomalous scattering in brominated DNA and RNA molecules, and importance of crystal orientation in single- and multiple-wavelength anomalous diffraction phasing

X-ray absorption, refraction and resonant scattering tensors in selenated protein crystals: implications for data collection strategies in macromolecular crystallography

Exploiting X-ray induced anisotropic lattice changes to improve intensity extraction in protein powder diffraction: Application to heavy atom detection

Molecular envelopes derived from protein powder diffraction Molecular envelopes derived from protein powder diffraction data

Using X-ray absorption spectra to monitor specific radiation damage to anomalously scattering atoms in macromolecular crystallography

Instrument-independent specification of the diffraction geometry and polarization state of the incident X-ray beam

Directed evolution of the suicide protein O⁶-alkylguanine-DNA alkyltransferase for increased reactivity results in an alkylated protein with exceptional stability

Preliminary crystallographic analysis of two oligomerization-deficient mutants of the aerolysin toxin, H132D and H132N, in their proteolyzed forms

Crystallization and halide phasing of the C-terminal domain of human KIN17

Powder diffraction studies on proteins: An overview of data collection approaches

Crystal Structure of the PP2A Phosphatase Activator: Implications for Its PP2A-Specific PPIase Activity

Features of the secondary structure of a protein molecule from powder diffraction data

Intensity statistics of Friedel opposites

Modelling and refining site-specific radiation damage in SAD/MAD phasing

Crystal structure of yeast YER010Cp, a knotable member of the RraA protein family

Synthesis and structure of dipyrido-1,4-dithiins

Development of powder diffraction methods for macromolecular crystallography

A Polymorph Lost and Found: The High-Temperature Crystal Structure of Pentacene