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Alke Fink

Cette personne a quitté l’EPFL

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Publications associées (48)

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Characterization of PEI-coated superparamagnetic iron oxide nanoparticles for transfection: Size distribution, colloidal properties and DNA interaction

Heinrich Hofmann, Alke Fink, Benedikt Steitz

Superparamagnetic iron oxide nanoparticles (SPIONs) were coated with polyethylenimine. Here, we briefly describe the synthesis as well as DNA:PEI:SPION complexes and the characterization of the compounds according to their particle size, zeta-potential, mo ...

2007

Superparamagnetic iron oxide nanoparticles (SPIONs): from synthesis to in vivo studies - A summary of the synthesis, characterization, in vitro and in vivo investigations of SPIONs with particular focus on surface and colloidal properties

Heinrich Hofmann, Alke Fink

In this work, we present a short summary of the synthesis and characterization of superparamagnetic iron oxide nanoparticles and their behavior in vitro and in vivo. Therefore, we have used various characterization techniques to deduce the physical particl ...

2007

MULTIFUNCTIONALIZED SPIONs FOR NUCLEAR TARGETING: CELL UPTAKE AND GENE EXPRESSION

Heinrich Hofmann, Alke Fink

Superparamagnetic iron oxide nanoparticles (SPIONs) are used in many biological applications, which necessitate intracellular targeting. Here, we investigate intracellular localization and gene expression in HeLa cells after treatment with functionalized S ...

World Scientific Publ Co Pte Ltd2014

Superparamagnetic Nanoparticles as a Powerful Systems Biology. Characterization Tool in the Physiological Context

Jeffrey Alan Hubbell, Heinrich Hofmann, Alke Fink, Marc Moniatte, Benedikt Steitz, Andrija Finka

Recently, functionalized superparamagnetic iron oxide nanoparticles (SPIONs) have been utilized for protein separation and therapeutic delivery of DNA and drugs. The development of new methods and tools for the targeting and identification of specific biom ...

Wiley-Blackwell2008

Methodology description for detection of cellular uptake of PVA coated superparamagnetic iron oxide nanoparticles (SPION) in synovial cells of sheep

Heinrich Hofmann, Alke Fink, Mathieu Chastellain

The detection of superparamagnetic iron oxide nanoparticles (SPION) in synoviocytes is reported. Synoviocytes were incubated for 2, 12, 24 and 48 h with 1.5 mg/ml of PVA coated SPION under the influence of magnets (12 h). Particles were well tolerated by t ...

2005

Development of biocompatible functionalized superparamagnetic iron oxide nanoparticles for human cancer cell uptake and intracellular delivery

Heinrich Hofmann, Alessio Ferrari, Alke Fink, Mathieu Chastellain

2005

Particle Size Distribution Measurements of Manganese-Doped ZnS Nanoparticles

Heinrich Hofmann, Alke Fink

We performed particle size and particle size distribution measurements for L-cysteine-stabilized ZnS/Mn nanoparticles in the size region below 10 nm. For this we applied transmission electron microscopy (TEM), analytical ultracentrifugation (AUC), dynamic ...

2009

Exposure to super-paramagnetic iron oxide nanoparticles reduces dendritic cell-dependent antigen-specific T-cell proliferation

Heinrich Hofmann, Alke Fink

Swiss Medical Publishers Ltd / EMH Swiss Medical Publishers2009

Surface charge of polymer coated SPIONs influences the serum protein adsorption, colloidal stability and subsequent cell interaction in vitro

Alke Fink, Marc Moniatte

It is known that the nanoparticle-cell interaction strongly depends on the physicochemical properties of the investigated particles. In addition, medium density and viscosity influence the colloidal behaviour of nanoparticles. Here, we show how nanoparticl ...

Royal Soc Chemistry2013

Safety management of nanomaterials

Thierry Meyer, Arnaud Magrez, Alke Fink, Amela Groso

2011

NanoSafe III: A User Friendly Safety Management System for Nanomaterials in Laboratories and Small Facilities

Thierry Meyer, Alke Fink, Anna Maria Novello, Elina Buitrago

Research in nanoscience continues to bring forward a steady stream of new nanomaterials and processes that are being developed and marketed. While scientific committees and expert groups deal with the harmonization of terminology and legal challenges, risk ...

2021

Intraarticular application of superparamagnetic nanoparticles and their uptake by synovial membrane—an experimental study in sheep

Heinrich Hofmann, Alke Fink, Benedikt Steitz, Mathieu Chastellain

A superparamagnetic iron oxide nanoparticle, coated with polyvinyl alcohol, (PVA-SPION) and its fluorescently functionalized analogue (amino-PVA-Cy3.5-SPION) were compared in vivo as proof of principle for future use in magnetic drug targeting in inflammat ...

2005

In vivo labelling of resting monocytes in the reticuloendothelial system with fluorescent iron oxide nanoparticles prior to injury reveals that they are mobilized to infarcted myocardium

Heinrich Hofmann, Alke Fink, Jean-Noël Hyacinthe

To evaluate the feasibility of loading resting monocytes/macrophages by intravenous (i.v.) injection of fluorescent iron oxide nanoparticles prior to injury and tracking of these cells in the very same animal to myocardial infarction (MI) by magnetic reson ...

Oxford University Press2010

Gene Expression in Synovial Membrane Cells After Intraarticular Delivery of Plasmid-Linked Superparamagnetic Iron Oxide Particles—A Preliminary Study in Sheep

Heinrich Hofmann, Alke Fink, Benedikt Steitz

This study evaluated in vivo gene deliver y and subsequent gene expression within cells of the synovium in the presence of static and pulsating magnetic field application following intraar ticular injection of super paramagnetic iron oxide nanopar ticles l ...

2006

Application of pulsed-magnetic field enhances non-viral gene delivery in primary cells from different origins

Heinrich Hofmann, Alke Fink, Benedikt Steitz

Primary cell lines are more difficult to transfect when compared to immortalized/transformed cell lines, and hence new techniques are required to enhance the transfection efficiency in these cells. We isolated and established primary cultures of synoviocyt ...

2008

INJECTABLE SUPERPARAMAGNETIC NANOPARTICLES FOR TREATMENT BY HYPERTHERMIA AND USE FOR FORMING AN HYPERTHERMIC IMPLANT

Heinrich Hofmann, Alke Fink, Mathieu Chastellain

The injectable formulation for treatment by hyperthermia comprises a liquid carrier and heat-generating superparamagnetic iron oxide nanoparticles having a mean diameter not greater than 20 nm. Said injectable formulation is able to form in-situ a hyperthe ...

2006

Recent advances in nano-bio-interactions: Editorial

Paul Bowen, Sami Rtimi, Alke Fink

ELSEVIER SCIENCE BV2019

Uptake and Biocompatibility of Functionalized Poly(vinylalcohol) Coated Superparamagnetic Maghemite Nanoparticles by Synoviocytes In Vitro

Heinrich Hofmann, Alke Fink, Benedikt Steitz

Superparamagnetic iron oxide nanoparticles (SPION) were coated with either Polyvinyl alcohol (PVA) or Vinyl alcohol/vinyl amine copolymer and further functionalized with the fluorochromes Cy3.5 or Texas Red. A colloidally stable suspension of nanoparticles ...

2006

Local moderate magnetically induced hyperthermia using an implant formed in situ in a mouse tumor model

Heinrich Hofmann, Alke Fink

Purpose: We investigate a new heat delivery technique for the local treatment of solid tumors. The technique involves injecting a formulation that solidifies to form an implant in situ. This implant entraps superparamagnetic iron oxide nanoparticles (SPION ...

Taylor & Francis2009

Superparamagnetic iron oxide Nanoparticles for biomedical application: a focus on PVA as a coating

Heinrich Hofmann, Alke Fink, Mathieu Chastellain

2003