Publications de Karen Martinez

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NOVELTY - Determining a receptor-ligand interaction involves repeatedly contacting a cell comprising a receptor, with a ligand, of the receptor in which the ligand has a fluorescence labeling group and binds reversibly to the receptor under conditions in w ...

The lateral organization of a prototypical G protein-coupled receptor, the neurokinin-1 receptor (NK1R), was investigated in living cells by fluorescence resonance energy transfer (FRET) microscopy, taking advantage of the recently developed acyl carrier p ...

Cell-membrane sheets suitable for in-vitro functional fluorescence studies have been prepd. by direct detachment from cell membranes using poly-L-lysine-coated glass slides. The resulting transferred planar membranes conserve the compn. as well as most pro ...

G protein-coupled receptors (GPCRs) constitute a large class of seven transmembrane proteins, which bind selectively agonists or antagonists with important consequences for cellular signaling and function. Comprehension of the mol. details of ligand binding is important for the understanding of receptor function and in turn for the design and development of novel therapeutic compds. Here we show how ligand-receptor interaction can be investigated in situ with high sensitivity on sensor surfaces by total int

We report on an in vivo single-mol. study of the signaling kinetics of G protein-coupled receptors (GPCR) performed using the neurokinin 1 receptor (NK1R) as a representative member. The NK1R signaling cascade is triggered by the specific binding of a fluo ...

With the reversible sequential (ReSeq) binding assay, we present a novel approach for the ultrasensitive profiling of receptor function in single living cells. This assay is based on the repetitive application of fluorescent ligands that have fast assocn.- ...

Supported cell-membrane sheets are promising in vitro systems to investigate the properties of membranes with native protein/lipid composition, in particular their sub-compartmentalization and the differential localization of proteins associated to them. W ...

Nanometer light source: Interaction between high-affinity binding sites on lipid-coated nanocrystals (NCs) and different proteins enables the controlled formation of supramol. nanostructures on micropatterned surfaces. The NCs serve as local light sources ...

Fluorescence resonance energy transfer (FRET) is a powerful technique to reveal interactions between membrane proteins in live cells. Fluorescence labeling for FRET is typically performed by fusion with fluorescent proteins (FP) with the drawbacks of a lim ...

Karen Martinez

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Determining a receptor-ligand interaction useful in e.g. drug screening involves repetitive application of reversibly binding fluorescent ligands with fast association-dissociation kinetics

FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells

Supported cell-membrane sheets for functional fluorescence imaging of membrane proteins

Ligand Binding to G Protein-Coupled Receptors in Tethered Cell Membranes

Kinetics of the initial steps of G protein-coupled receptor-mediated cellular signaling revealed by single-molecule imaging

Reversible sequential-binding probe receptor-ligand interactions in single cells

Monitoring the Diffusion of Single Heterotrimeric G Proteins in Supported Cell-membrane Sheets Reveals their Partitioning into Microdomains

Lipid-coated nanocrystals as multifunctionalized luminescent scaffolds for supramolecular biological assemblies

Covalent labeling of cell-surface proteins for in-vivo FRET studies