Publications de Nicolas Demierre

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This thesis reports on the integration of continuous-flow cell separation method for lab-on-a-chip applications. Cell separation methods are widely used in biology to prepare samples prior to analysis. There is a need for a highly sensitive separation meth ...

There is great interest in highly sensitive separation methods capable of quickly isolating a particular cell type within a single manipulation step prior to their analysis. We present a cell sorting device based on the opposition of dielectrophoretic forc ...

We present a device capable of electrical cell lysis and evaluation of lysis efficiency in continuous flow using dielectrophoretic cell sorting. We use a combination of AC electrical fields and so-called liquid electrodes to avoid bubble creation at the el ...

Impedance spectroscopy is a powerful tool for label-free analysis and characterisation of living cells. In this work, we achieved the detection of Babesia bovis infected red blood cells using impedance spectroscopy on a microfabricated flow cytometer. The ...

We have fabricated a microfluidic device based on a novel geometrical arrangement of lateral metal electrodes and a patterned insulator. Our device combines the concept of insulator-based “electrodeless” dielectrophoresis with multiple frequencies to achie ...

Cell cycle synchronization is an important tool for the study of the cell division stages and signalling. It provides homogeneous cell cultures that are of importance to develop and improve processes such as protein synthesis and drug screening. The main a ...

Dielectrophoresis is a method that has demonstrated great potential in cell discrimination and isolation. In this study, the dielectrophoretic sorting of normal and Babesia bovis infected erythrocytes was performed using a microfabricated flow cytometer. S ...

This paper reports on a novel method for on-chip continuous separation of divid- ing and non-dividing cells based on differences in their dielectric properties. By means of two opposite dielectrophoretic force fields at multiple frequencies, the two populations of cells flowing through the microfluidic device are focused towards distinct equilibrium positions, which can be correlated to their cell cycle.

Using the concept of insulator-based "electrodeless" dielectrophoresis, we present a novel geometry for shaping electric fields to achieve lateral deviation of particles in liquid flows. The field is generated by lateral planar metal electrodes and is guid ...

We present a channel geometry that allows for clean switching between different inlets of a microchip without any contamination of the inlets or the downstream flow. We drive this virtual valve with a pneumatic pressure setup that minimizes disturbance of ...

This paper reports on a new microfluidic chip designed for cell electrofusion. A pair of cells is brought into close contact by means of opposite dielectrophoretic forces and they are subsequently fused by applying a short electrical pulse.

We present a particle-sorting device based on the opposition of dielectrophoretic forces. The forces are generated by an array of electrode chambers located in both sidewalls of a main flow channel. Particles with different dielectric response perceive dif ...

We present a microfluidic device where micro- and nanoparticles can be continuously functionalized in flow. This device relies on an element called particle exchanger, which allows for particles to be taken from one medium and exposed to some reagent while ...

Microfluidic system comprising a space (0207) for containing a liquid and at least one lateral chamber (0201) in communication with said space, said lateral chamber containing a metal electrode (0204). The lateral chamber and the space are designed to be f ...

We describe a multiplexing technology, named Evalution, based on novel digitally encoded microparticles in microfluidic channels. Quantitative multiplexing is becoming increasingly important for research and routine clinical diagnostics, but fast, easy-to- ...

Nicolas Demierre

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Continuous-flow separation of cells in a lab-on-a-chip using "liquid electrodes" and multiple-frequency dielectrophoresis

A miniaturized continuous dielectrophoretic cell sorter and its applications

Continuous-flow electrical lysis device with integrated control by dielectrophoretic cell sorting

Label-free detection of Babesia bovis infected red blood cells using impedance spectroscopy on microfabricated flow cytometer

Focusing and continuous separation of cells in a microfluidic device using lateral dielectrophoresis

Tracking and synchronization of the yeast cell cycle using dielectrophoretic opacity

Dielectrophoretic sorting on a microfabricated flow cytometer: Label free separation of Babesia bovis infected erythrocytes

Microfluidic device for continuous dielectrophoretic separation of cells in division

Characterization and optimization of liquid electrodes for lateral dielectrophoresis

A virtual valve for smooth contamination-free flow switching

Continuous flow cell trapping and hybridoma-cell production on-chip using "liquid electrodes"

Continuous separation of cells by balanced dielectrophoretic forces at multiple frequencies

Dielectrophoresis-based particle exchanger for the manipulation and surface functionalization of particles

A simple pneumatic setup for driving microfluidics

Continuous separation of cells in a microfluidic device using lateral dielectrophoresis

Apparatus for manipulating, modifying and characterizing particles in a micro channel

Rapid, Sensitive and Real-Time Multiplexing Platform for the Analysis of Protein and Nucleic-Acid Biomarkers

Creating structures at the scale of cells on the fly: gentle single cell entrapment on chip

Liquid Electrodes and Multiple-Frequency Dielectrophoresis for studying bacterial persistence to antibiotics