Publications de Michel Prudent

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Metal ions are essential elements in all living systems. Their detections and identifications in biological processes require different methodologies. Mass spectrometry is one of the commonly used techniques in bioinorganic analyses to fulfill these requir ...

We propose methods to quantitatively calculate the fluctuation rate of red blood cells with nanometric axial and millisecond temporal sensitivity at the single-cell level by using time-lapse holographic cell imaging. For this quantitative analysis, cell me ...

A combination of an immuno-affinity enrichment strategy and sensitive amperometric read-out was implemented in a point-of-care platform intended for bacterial infection analysis. Bacterial cells, selectively captured and enriched from complex matrices thro ...

The present work shows that the electrochemical properties of electrospray ionization (ESI) can be used to add functions to the process. As example, we show how the choice of the electrode material can be used to study interactions between metal ions and b ...

alpha-Synuclein (alpha-syn) is a 140-amino acid protein that plays a central role in the pathogenesis of Parkinson's disease (PD) and other synucleinopathies. However, the molecular determinants that are responsible for triggering and/or propagating alpha- ...

Background and Objectives: Red blood cells (RBCs) suffer from lesions during cold storage, depending in part on their ability to counterbalance oxidative stress by activating their antioxidant defence. The aim of this study was to monitor the antioxidant p ...

Bloodstream infections rank among the most serious causes of morbidity and mortality in hospitalized patients, partly due to the long period (up to one week) required for clinical diagnosis. In this work, we have developed a sensitive method to quickly and ...

Hydrogen peroxide generation at a liquid|liquid interface occurs with a yield of 20 % with respect to the concentration of reducing agent (decamethylferrocene). The liquid|liquid interface supplies electrons from the reducing agent and protons from the aqu ...

The electrochemically induced mass spectrometric tagging of cysteines by substituted hydroquinones was studied for peptides in a classical electrospray solvent (i.e., MeOH/H2O/AcOH 50/49/1). The tagging efficiency was tested with different hydroquinone com ...

Biphasic electrospray ionization (BESI) mass spectrometry is achieved by using a dual-channel microsprayer, where channels filled with different immiscible phases meet at the Taylor cone. Two types of interfacial complexation reactions have been studied: t ...

Single metal ion-phospholipid complexes are observed in biphasic electrospray ionization mass spectrometry (BESI-MS) using a dual-channel microsprayer. Such a microsprayer makes it possible to put into contact two immiscible liquids within the Taylor cone. ...

Two peptides known to interact with receptors embedded in cell membranes, angiotensin III (AngIII) and Leuenkephalin(LeuEnk), were studied electrochemically at the interface formed between two immiscible electrolyte solutions modified by an adsorbed monola ...

Cysteine-containing peptide oxidation was studied both by using an inert platinum electrode and a sacrificial electrode (copper or zinc) generating metallic ions in electrospray ionization mass spectrometry (ESI-MS). Using peptides containing one, two and ...

Multitrack electrospray chips (MTEC) were fabricated by UV-photoablation of polyethylene terephthalate (PET) substrates. They are composed of an array of up to six microchannels that are successively used as electrospray ionization (ESI) emitters for mass ...

The interaction of copper ions with peptides was investigated by electrospray mass spectrometry. Two electrospray micro-emitters were compared, the first one with a platinum electrode using a copper(II) electrolyte solution containing a peptide sample, and ...

NOVELTY - A plate comprises an electroconductive substrate (1) at least partially covered with sintered nanoparticles (2), deposited as an array of individual spots, for use as a sorbing phase for a sample, and for supporting ionization of sorbed sample mo ...

Copper-b-amyloid 16 (Aβ16) complexes were investigated by electrospray ionization mass spectrometry (ESI-MS). Copper(I) and (II) complexes were formed on-line in a microchip electrospray emitter by using a sacrificial copper electrode as the anode in posit ...

Electrochemical or photo-electrochemical reactions in both electrospray ionization and laser desorption ionization are discussed stressing the role of the electrode reaction in influencing the ionization process. In particular, upon application of a high v ...

Herein, we present the large scale fabrication of carbon nanotubes (CNT) electrodes supported on flexible polymeric sheets by subsequent multilayer inkjet printing of a silver layer for electrical connection, CNT layers as active electrode material and an ...

Phosphopeptides tagging reactions by dinuclear zinc(II) complexes (1,3-bis[bis(2-pyridylmethyl)amino]-propan- 2-olato dizinc(II)3+, called tag) were performed with a dual-channel microsprayer in electrospray ionization mass spectrometry. The reaction is fi ...

Michel Prudent

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Electrospray ionization mass spectrometry : electrochemistry and on-chip reactions for bioinorganic analyses

Quantification of stored red blood cell fluctuations by time-lapse holographic cell imaging

Immuno-affinity Amperometric Detection of Bacterial Infections

About the Electrospray Ionization Source in Mass Spectrometry: Electrochemistry and On-chip Reactions

Phosphorylation of alpha-Synuclein at Y125 and S129 Alters Its Metal Binding Properties: Implications for Understanding the Role of alpha-Synuclein in the Pathogenesis of Parkinson's Disease and Related Disorders

The antioxidant capacity of erythrocyte concentrates is increased during the first week of storage and correlated with the uric acid level

Sensitive and fast identification of bacteria in blood samples by immunoaffinity mass spectrometry for quick BSI diagnosis

H2O2 generation by decamethylferrocene at a liquid | liquid interface

On-Line Counting of Cysteine Residues in Peptides during Electrospray Ionization by Electrogenerated Tags and Their Application to Protein Identification

Biphasic Electrospray Ionization for the Study of Interfacial Complexes

Formation and study of single metal ion–phospholipid complexes in biphasic electrospray ionization mass spectrometry

Peptide-Phospholipid Complex Formation at Liquid-Liquid Interfaces

The role of copper in cysteine oxidation: study of intra- and inter-molecular reactions in mass spectrometry

Multitrack electrospray chips

On-line Electrogeneration of Copper-Peptide Complexes in Microspray Mass Spectrometry

Plate for matrix-assisted laser desorption ionization mass spectrometry, comprises electroconductive substrate covered with sintered nanoparticles, deposited as array of spots, for supporting ionization of sorbed sample molecules

Copper(I) and copper(II) binding to β-amyloid 16 (Aβ16) studied by electrospray ionization mass spectrometry

Electrochemical reactions and ionization processes

Large scale inkjet-printing of carbon nanotubes electrodes for antioxidant assays in blood bags

Microfabricated Dual Sprayer for On-Line Mass Tagging of Phosphopeptides