Publications de Wolf-Peter Ulrich

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A generic method is described for the reversible immobilization of polyhistidine-bearing polypeptides and proteins on attenuated total reflecting (ATR) sensor surfaces for the detection of biomol. interactions by FTIR spectroscopy. Nitrilotriacetic acid (NTA) groups are covalently attached to self-assembled monolayers of either thioalkanes on gold films or mercaptosilanes on silicon dioxide films deposited on germanium internal reflection elements. Complex formation between Ni2+ ions and NTA groups activate

The outer membrane protein OmpF from Escherichia coli is a member of a large family of b-barrel membrane proteins. Some, like OmpF, are pore-forming proteins while others are active transporters or enzymes. We have previously shown that the receptor-binding domain (R-domain) of the toxin colicin N binds with high affinity to OmpF reconstituted into tethered lipid bilayers on gold electrodes. The binding can be measured by surface plasmon resonance (SPR) and ion channel blockage (impedance spectroscopy, IS).

High signal-to-noise Fourier transform IR (FTIR) spectra of the 5-hydroxytryptamine (serotonin) receptor (5-HT3R) and the nicotinic acetylcholine receptor (nAChR) were obtained by microscope FTIR spectroscopy using micrometer-sized, fully hydrated protein films. Because this novel procedure requires only nanogram quantities of membrane proteins, which is 4-5 orders of magnitude less than the amt. of protein typically used for conventional FTIR spectroscopy, it opens the possibility to access the structure a

Hydrophobins self assemble into amphipathic films at hydrophobic-hydrophilic interfaces. These proteins are involved in a broad range of processes in fungal development. We have studied the conformational changes that accompany the self-assembly of the hydrophobin SC3 with polarization-modulation IR reflection absorption spectroscopy, attenuated total reflection Fourier transform IR spectroscopy, and CD, and related them to changes in morphol. as obsd. by electron microscopy. Three states of SC3 have been s

Monolayers of gramicidin A, pure and in mixts. with dimyristoylphosphatidylcholine (DMPC), were studied in situ at the air/H2O and air/D2O interfaces by polarization-modulated IR reflection absorption spectroscopy (PM-IRRAS). Simulations of the entire set of amide I absorption modes were also performed, using complete parameter sets for different conformations based on published normal mode calcns. The structure of gramicidin A in the DMPC monolayer could clearly be assigned to a b6.3 helix. Quant. anal. of

We show that it is possible to immobilize membrane proteins uniformly and reversibly as self-assembled (sub)monolayers on nitrilotriacetic acid-covered sensor surfaces via hexahistidine sequences present either in the protein or in lipid membranes. Fourier transform IR spectra of such self-assembled (sub)monolayers deliver important structural information of the membrane proteins and are suited to screen the function of cellular receptors. [on SciFinder (R)]

The invention relates to an isotropic cellulose film deposited on an inorg. support layer. The invention also relates to a process for manufg. such cellulose films as well as an anal. cell comprising such deposited films. The cellulose film is particularly ...

Gating of an artificial receptor was monitored for the first time by impedance spectroscopy in tethered lipid bilayer. Subnanomolar concns. of antibody were detected by modulation of the channel activity. The principle of gating by an antibody is particularly attractive because of its general character, and thus opens new possibility of immunosensing. [on SciFinder (R)]

Wolf-Peter Ulrich

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Reversible immobilization of peptides: Surface modification and in situ detection by attenuated total reflection FTIR spectroscopy

Stable self-assembly of a protein engineering scaffold on gold surfaces

Downscaling Fourier Transform Infrared Spectroscopy to the Micrometer and Nanogram Scale: Secondary Structure of Serotonin and Acetylcholine Receptors

Self-assembly of the hydrophobin SC3 proceeds via two structural intermediates

Polarization-modulated FTIR spectroscopy of lipid/gramicidin monolayers at the air/water interface

Controlled Immobilization of Membrane Proteins to Surfaces for Fourier Transform Infrared Investigations

Cellulose model surface

Immunosensing by a synthetic ligand-gated ion channel