Publications de Samuel Terrettaz

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This paper presents a switched-capacitor (SC) current integrator circuit for impedance measurement of tethered bilayer lipid membrane (tBLM) biosensors. The circuit comprises a small number of high performance components enabling enhanced experimental flex ...

The outer membrane protein OmpF from Escherichia coli is a member of a large family of b-barrel membrane proteins. Some, like OmpF, are pore-forming proteins while others are active transporters or enzymes. We have previously shown that the receptor-binding domain (R-domain) of the toxin colicin N binds with high affinity to OmpF reconstituted into tethered lipid bilayers on gold electrodes. The binding can be measured by surface plasmon resonance (SPR) and ion channel blockage (impedance spectroscopy, IS).

Tethered lipid bilayers, containing a transmembrane synthetic ligand-gated ion channel (SLIC), have been formed on gold surfaces. The SLIC was designed as a highly selective receptor and reporter protein to detect antibodies in whole blood, which are of im ...

A Ca2+ sensor based on the admittance change of synthetic membranes supported on derivatized silicon electrodes is reported. The ion-sensitive membranes consist of mixed monolayers of phospholipid and the Ca2+-ligand ETH 1001. The electrodes are characterized electrochem. and their sensor performances are described. Through ion complexation by the neutral ligand, elec. charges are incorporated in the low-dielec.-const. environment of the sensitive layer. The no. of charged complexes and thus the membrane ad

The human estrogen receptor alpha (ER!) mediates the proliferative action of hormones in breast cancer cells by regulating the expression of target genes to control cellular functions. Current methodologies do not permit a real-time assessment of these pro ...

The attachment of a tetradecyl chain to the ionophore 18-crown-6 leads to a stronger interaction with a supported lipid monolayer. Some C14-18-crown-6 mols. introduced in the aq. phase are incorporated in the phospholipid membrane. This process is used to produce ionophore contg. lipid monolayers of detd. compn. at the surface of an electrode. The ionophore content and the sensitivity to K+ of these layers are detd. by capacitance measurements. [on SciFinder (R)]

The specific recognition reaction between cholera toxin (ChTo) and the ganglioside GM1 was investigated in situ on planar supported lipid layers using simultaneous surface plasmon resonance (SPR) and electrochem. impedance measurements. The lipid monolayers contg. different amts. of GM1 were formed on alkanethiol-coated gold surfaces by a new lipid/detergent diln. technique. The formation of the layers was investigated using both SPR and impedance measurements. While the optical properties of the different

A review. The function of biol. important ion channels can be measured in supported lipid membranes by impedance spectroscopy. This approach offers substantial advantages over traditional electrophysiol. measurements. In this article, we present an overvie ...

A method is presented to form gold-electrode-tethered lipid bilayers with exceptionally high elec. resistances. Elec. impedance spectroscopy is used to monitor the bilayer incorporation of a ligand-gated ion channel protein and the modulation of its channel activity by the selective binding of an antibody. Due to the low defect d. of the tethered membrane, the effect of a few channels can be resolved thus opening the way to single-channel expts. on this highly stable and versatile platform. In turn, a minut

A review. The efficient anal. of the function of ion channels is an important task in many different fields ranging from (bio)analytics and drug-screening to nanoelectronics. Here, the modulation of the channel activity of a synthetic ligand gated ion chan ...

Gating of an artificial receptor was monitored for the first time by impedance spectroscopy in tethered lipid bilayer. Subnanomolar concns. of antibody were detected by modulation of the channel activity. The principle of gating by an antibody is particularly attractive because of its general character, and thus opens new possibility of immunosensing. [on SciFinder (R)]

Alkanethiolates have been widely used as chemisorbates to modify gold surfaces, in spite of their relatively poor oxidative stability. We introduce gold-chemisorbing block copolymers bearing an anchoring block of poly(propylene sulfide) (PPS), selected in the expectation of greater stability. These materials offer a more robust approach to surface modification of gold. As an example, a triblock copolymer with poly(ethylene glycol) (PEG) was selected, with the goal of minimizing biol. adsorption and adhesion

Samuel Terrettaz

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Real-time high-sensitivity impedance measurement interface for tethered BLM biosensor arrays

Stable self-assembly of a protein engineering scaffold on gold surfaces

A synthetic membrane protein in tethered lipid bilayers for immunosensing in whole blood

Calcium(2+)-sensitive monolayer electrodes

Monitoring proliferative activities of hormone-like odorants in human breast cancer cells by gene transcription profiling and electrical impedance spectroscopy

Determination of the surface concentration of crown ethers in supported lipid membranes by capacitance measurements

Protein binding to supported lipid membranes: investigation of the cholera toxin-ganglioside interaction by simultaneous impedance spectroscopy and surface plasmon resonance

Investigating the function of ion channels in tethered lipid membranes by impedance spectroscopy

Highly electrically insulating tethered lipid bilayers for probing the function of Ion channel proteins

Impedance spectroscopy of ion channels in tethered lipid bilayers

Immunosensing by a synthetic ligand-gated ion channel

Probing the function of ionotropic and G protein-coupled receptors in surface-confined membranes

Chemisorbed poly(propylene sulphide)-based copolymers resist biomolecular interactions