Publications de Henning Paul-Julius Stahlberg

Correlative light and electron microscopy for human brain and other biological models

Henning Paul-Julius Stahlberg, Amanda Jennifer Lewis, Domenic Burger, Marta Di Fabrizio

Correlative light and electron microscopy (CLEM) combines light microscopy, for identifying a target via genetic labels, dyes, antibodies and morphological features, with electron microscopy, for analyzing high-resolution subcellular ultrastructures. Here, ...

NATURE PORTFOLIO2025

Structural basis of SIRT7 nucleosome engagement and substrate specificity

Henning Paul-Julius Stahlberg, Beat Fierz, Dongchun Ni, Babatunde Edukpe Ekundayo, Carlos Moreno Yruela

Chromatin-modifying enzymes target distinct residues within histones to finetune gene expression profiles. SIRT7 is an NAD+-dependent deacylase often deregulated in cancer, which deacetylates either H3 lysine 36 (H3K36) or H3K18 with high specificity withi ...

NATURE PORTFOLIO2025

A Comparative Analysis of STEM Phase Retrieval Techniques: Evaluating Transfer of Information and Dose Efficiency

Henning Paul-Julius Stahlberg

Oxford University Press (OUP)2025

Identification of a binding site for small molecule inhibitors targeting human TRPM4

Henning Paul-Julius Stahlberg, Dongchun Ni, Babatunde Edukpe Ekundayo

Transient receptor potential (TRP) melastatin 4 (TRPM4) protein is a calcium-activated monovalent cation channel associated with various genetic and cardiovascular disorders. The anthranilic acid derivative NBA is a potent and specific TRPM4 inhibitor, but ...

Nature Portfolio2025

Arrestin recognizes GPCRs independently of the receptor state

Henning Paul-Julius Stahlberg, Dongchun Ni, Meltem Tatli

Only two nonvisual arrestins recognize many hundreds of different, intracellularly phosphorylated G protein-coupled receptors (GPCRs). Due to the highly dynamic nature of GPCR•arrestin complexes, the critical determinants of GPCR–arrestin recognition have ...

Proceedings of the National Academy of Sciences2025

Phase Contrast 4D-STEM Tomography of Thick Biological Specimens

Henning Paul-Julius Stahlberg

Oxford University Press (OUP)2025

Joint Ptychographic Tomography of Frozen Hydrated Proteins

Henning Paul-Julius Stahlberg

2024

Ultrastructural characterization of tau pathology in the human post‐mortem Alzheimer’s disease brain

Henning Paul-Julius Stahlberg, Amanda Jennifer Lewis

Neuropathological tau tangles in Alzerheimer’s disease (AD) have been roughly characterized into pre‐tangles, mature tangles and ghost tangles. However, to date, their maturation in the human brain is poorly understood. Here, we aimed to define the structu ...

Wiley2024

Structure-function analysis of the cyclic β-1,2-glucan synthase from Agrobacterium tumefaciens

Henning Paul-Julius Stahlberg, Dongchun Ni

The synthesis of complex sugars is a key aspect of microbial biology. Cyclic beta-1,2-glucan (C beta G) is a circular polysaccharide critical for host interactions of many bacteria, including major pathogens of humans (Brucella) and plants (Agrobacterium). ...

Nature Portfolio2024

Computational drug development for membrane protein targets

Henning Paul-Julius Stahlberg, Pierre Vogel, Shuguang Yuan, Dongchun Ni, Babatunde Edukpe Ekundayo

The application of computational biology in drug development for membrane protein targets has experienced a boost from recent developments in deep learning-driven structure prediction, increased speed and resolution of structure elucidation, machine learni ...

2024

Distinct ultrastructural phenotypes of glial and neuronal alpha-synuclein inclusions in multiple system atrophy

Henning Paul-Julius Stahlberg, Amanda Jennifer Lewis, Domenic Burger, Marta Di Fabrizio, Carolin Böing

Multiple System Atrophy is characterized pathologically by the accumulation of alpha-synuclein (aSyn) into glial cytoplasmic inclusions (GCIs). The mechanism underlying the formation of GCIs is not well understood. In this study, correlative light and elec ...

2024

Low-dose cryo-electron ptychography of proteins at sub-nanometer resolution

Henning Paul-Julius Stahlberg, Kelvin Ka Ching Lau

Cryo-transmission electron microscopy (cryo-EM) of frozen hydrated specimens is an efficient method for the structural analysis of purified biological molecules. However, cryo-EM and cryo-electron tomography are limited by the low signal-to-noise ratio (SN ...

Nature Research2024

Modulation of ABCG2 Transporter Activity by Ko143 Derivatives

Henning Paul-Julius Stahlberg, Dongchun Ni

ABCG2 is a multidrug transporter that protects tissues from xenobiotics, affects drug pharmacokinetics, and contributes to multidrug resistance of cancer cells. Here, we present tetracyclic fumitremorgin C analog Ko143 derivatives, evaluate their in vitro ...

2024

Rapid iPSC inclusionopathy models shed light on formation, consequence, and molecular subtype of α-synuclein inclusions

Henning Paul-Julius Stahlberg, Amanda Jennifer Lewis

The heterogeneity of protein-rich inclusions and its significance in neurodegeneration is poorly understood. Standard patient-derived iPSC models develop inclusions neither reproducibly nor in a reasonable time frame. Here, we developed screenable iPSC “in ...

2024

Mouse α-synuclein fibrils are structurally and functionally distinct from human fibrils associated with Lewy body diseases

Henning Paul-Julius Stahlberg, Hilal Lashuel, Sonia Donzelli

The intricate process of α-synuclein aggregation and fibrillization holds pivotal roles in Parkinson’s disease (PD) and multiple system atrophy (MSA). While mouse α-synuclein can fibrillize in vitro, whether these fibrils commonly used in research to induc ...

2024

Author Correction: Fully co-factor-free ClearTau platform produces seeding-competent Tau fibrils for reconstructing pathological Tau aggregates

Henning Paul-Julius Stahlberg, Hilal Lashuel, Sergey Nazarov, Galina Limorenko, Rajasekhar Kolla, Meltem Tatli

Nature Research2024

Hierarchical Protofilament Intertwining Rules the Formation of Mixed‐Curvature Amyloid Polymorphs

Henning Paul-Julius Stahlberg, Giovanni Dietler, Serguei Sekatski, Francesco Simone Ruggeri, Jiangtao Zhou, Meltem Tatli

Amyloid polymorphism is a hallmark of almost all amyloid species, yet the mechanisms underlying the formation of amyloid polymorphs and their complex architectures remain elusive. Commonly, two main mesoscopic topologies are found in amyloid polymorphs cha ...

Wiley2024

Automated fiducial-based alignment of cryo-electron tomography tilt series in Dynamo

Henning Paul-Julius Stahlberg

With the advent of modern technologies for cryo-electron tomography (cryo-ET), high-quality tilt series are more rapidly acquired than processed and analyzed. Thus, a robust and fast-automated alignment for batch processing in cryo-ET is needed. While diff ...

CELL PRESS2024

Cryo-electron microscopy in color

Henning Paul-Julius Stahlberg

Cryo-electron microscopy with energy resolution, using the EELS-STEM method, allows researchers to identify the approximate locations of certain heavier atoms within single frozen, hydrated protein particles. ...

NATURE PORTFOLIO2024

Structural Basis of the Allosteric Inhibition of Human ABCG2 by Nanobodies

Henning Paul-Julius Stahlberg, Dongchun Ni

ABCG2 is an ATP-binding cassette transporter that exports a wide range of xenobiotic compounds and has been recognized as a contributing factor for multidrug resistance in cancer cells. Substrate and inhibitor interactions with ABCG2 have been extensively ...

Academic Press Ltd- Elsevier Science Ltd2023