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Rudolf Hovius

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Publications associées (34)

Acetylcholine Receptor Organization in Membrane Domains in Muscle Cells: EVIDENCE FOR RAPSYN-INDEPENDENT AND RAPSYN-DEPENDENT MECHANISMS

Rudolf Hovius, Horst Vogel, Jean-Manuel Segura, Joachim Piguet, Christoph Schreiter

Nicotinic acetylcholine receptors (nAChR) in muscle fibers are densely packed in the postsynaptic region at the neuromuscular junction. Rapsyn plays a central role in directing and clustering nAChR during cellular differentiation and neuromuscular junction ...

American Society for Biochemistry and Molecular Biology2011

Serotonin receptor diversity in the human colon: Expression of serotonin type 3 receptor subunits 5-HT3C, 5-HT3D, and 5-HT3E

Rudolf Hovius

Since the first description of 5-HT3 receptors more than 50 years ago, there has been speculation about the molecular basis of their receptor heterogeneity. We have cloned the genes encoding novel 5-HT3 subunits 5-HT3C, 5-HT3D, and 5-HT3E and have shown th ...

Wiley-Blackwell2010

Repetitive Reversible Labeling of Proteins at Polyhistidine Sequences for Single-Molecule Imaging in Live Cells

Rudolf Hovius, Horst Vogel, Emmanuel Guignet, Jean-Manuel Segura

Sensitive live-cell fluorescence microscopy and single-molecule imaging are severely limited by rapid photobleaching of fluorescent probes. Herein, we show how to circumvent this problem using a novel, generic labeling strategy. Small nickel-nitrilotriacet ...

2007

Fluorescent Epibatidine Agonists for Neuronal and Muscle-Type Nicotinic Acetylcholine Receptors

Rudolf Hovius, Horst Vogel, Jörg Grandl

Wiley-VCH Verlag GmbH2007

Modulation of proton-induced current fluctuations in the human nicotinic acetylcholine receptor channel

Rudolf Hovius, Horst Vogel, Jörg Grandl, Christophe Danelon

The nicotinic acetylcholine receptor (nAChR) is a ligand-gated ion channel that switches upon activation from a closed state to a full conducting state. We found that the mutation delta S268K, located at 12' position of the second transmembrane domain of t ...

2007

New conjugate of agonist ligand directed to ligand-gated ion channels of cysteine loop-family with chromophore, useful, e.g. in receptor proteins/their interaction with agonistic ligand and to study the effect of unlabelled ligands

Rudolf Hovius, Horst Vogel, Jörg Grandl

NOVELTY - Conjugate (I) of an agonist ligand directed to ligand-gated ion channels (LGICs) of the Cys loop-family with a chromophore, is new. USE - (I) is useful in the receptor proteins and/or their interaction with the agonistic ligand, where the interac ...

2007

Determining a receptor-ligand interaction useful in e.g. drug screening involves repetitive application of reversibly binding fluorescent ligands with fast association-dissociation kinetics

Rudolf Hovius, Horst Vogel, Marinela Gjoni Boillat, Jean-Manuel Segura, Christoph Schreiter

NOVELTY - Determining a receptor-ligand interaction involves repeatedly contacting a cell comprising a receptor, with a ligand, of the receptor in which the ligand has a fluorescence labeling group and binds reversibly to the receptor under conditions in w ...

2006

FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells

Rudolf Hovius, Horst Vogel, Jean-Manuel Segura, Kai Johnsson, Nathalie George

The lateral organization of a prototypical G protein-coupled receptor, the neurokinin-1 receptor (NK1R), was investigated in living cells by fluorescence resonance energy transfer (FRET) microscopy, taking advantage of the recently developed acyl carrier p ...

National Academy of Sciences2006

Functional asymmetry of transmembrane segments in nicotinic acetylcholine receptors

Rudolf Hovius, Horst Vogel, Jörg Grandl, Christophe Danelon

Nicotinic acetylcholine receptors are heteropentameric ion channels that open upon activation to a single conducting state. The second transmembrane segments of each subunit were identified as channel-forming elements, but their respective contribution in ...

2006

Covalent labeling of cell-surface proteins for in-vivo FRET studies

Rudolf Hovius, Horst Vogel, Jean-Manuel Segura, Kai Johnsson, Bruno Meyer, Nathalie George, Pedro Pascoal

Fluorescence resonance energy transfer (FRET) is a powerful technique to reveal interactions between membrane proteins in live cells. Fluorescence labeling for FRET is typically performed by fusion with fluorescent proteins (FP) with the drawbacks of a lim ...

2006

Investigating cellular signaling reactions in single attoliter vesicles

Rudolf Hovius, Horst Vogel, Horst Pick, Erwin Ilegems

Understanding cellular signaling mediated by cell surface receptors is key to modern biomedical research and drug development. The discovery of a growing no. of potential mol. targets and therapeutic compds. requires downscaling and accelerated functional ...

2005

Synthesis of nanoscopic optical fibers using lipid membranes as templates

Rudolf Hovius, Pierre Stadelmann, Horst Vogel, Dimitrios Stamou, Marinela Gjoni Boillat, Jean-Manuel Segura, Gopakumar Gopalakrishnan, Kurt Schenk, Cédric Gaillard

High-aspect-ratio, monocryst. Cd chloride nanowires doped with fluorescent CdS nanoparticles were synthesized using planar lipid membranes as templates. Cd2+ ions bound to the lipid bilayers (a) are released at low pH to the nanometer-sized, interlamellar ...

Wiley-VCH Verlag GmbH2005

Lipid-coated nanocrystals as multifunctionalized luminescent scaffolds for supramolecular biological assemblies

Rudolf Hovius, Ravindranathan Thampi, Horst Vogel

Nanometer light source: Interaction between high-affinity binding sites on lipid-coated nanocrystals (NCs) and different proteins enables the controlled formation of supramol. nanostructures on micropatterned surfaces. The NCs serve as local light sources ...

Wiley-VCH Verlag GmbH2005

Reversible sequential-binding probe receptor-ligand interactions in single cells

Rudolf Hovius, Horst Vogel, Marinela Gjoni Boillat, Jean-Manuel Segura, Christoph Schreiter

With the reversible sequential (ReSeq) binding assay, we present a novel approach for the ultrasensitive profiling of receptor function in single living cells. This assay is based on the repetitive application of fluorescent ligands that have fast assocn.- ...

2005

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