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Concept

Yeast flocculation

Publications associées (27)

Dielectrophoresis-based continuous-flow particle microreactor

This thesis mainly deals with the integration of a microfluidic system for the extraction of micrometric and submicrometric particles from a solution. Traditional methods take from several minutes to hours to separate particles from a solution. Microfluidi ...

EPFL2009

Structure and function of the molecular chaperone Hsp104 from yeast

Hilal Lashuel

The molecular chaperone Hsp104 plays a central role in the clearance of aggregates after heat shock and the propagation of yeast prions. Hsp104's disaggregation activity and prion propagation have been linked to its ability to resolubilize or remodel prote ...

Wiley-Blackwell2009

Focusing and continuous separation of cells in a microfluidic device using lateral dielectrophoresis

Philippe Renaud, Thomas Michael Braschler, Nicolas Demierre, Randoald Müller

We have fabricated a microfluidic device based on a novel geometrical arrangement of lateral metal electrodes and a patterned insulator. Our device combines the concept of insulator-based “electrodeless” dielectrophoresis with multiple frequencies to achie ...

Elsevier2008

A new method for protein labeling with small molecules based on acyl carrier protein

Nathalie George

A new method for the covalent and specific labeling of fusion proteins of carrier proteins (CPs) with small organic molecules has been developed in this work. This technology combines the convenience of expressing genetically tagged reporter proteins with ...

EPFL2006

Crystal structure of yeast YER010Cp, a knotable member of the RraA protein family

We present here the structure of Yer0l0c protein of unknown function, solved by Multiple Anomalous Diffraction and revealing a common fold and oligomerization state with proteins of the regulator of ribonuclease activity A (RraA) family. In Escherichia col ...

2005

Gentle cell trapping and release on a microfluidic chip by in situ alginate hydrogel formation

Philippe Renaud, Thomas Michael Braschler, Lynda Metref

Microfluidic devices are increasingly used to perform biological experiments on a single-cell basis. However, long-term stability of cell positions is still an issue. A novel biocompatible method for cell entrapment and release on a microchip is presented. ...

2005

AtSGP1, AtSGP2 and MAP4K alpha are nucleolar plant proteins that can complement fission yeast mutants lacking a functional SIN pathway

Viesturs Simanis, Andrea Krapp

In the fission yeast Schizosaccharomyces pombe, the onset of septum formation is signalled via the septation initiation network (SIN) involving several protein kinases and a GTPase. Arabidopsis thaliana and Brassica napus proteins homologous to fission yea ...

2004