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Concept

Endoplasmic-reticulum-associated protein degradation

Publications associées (8)

Autorepression of yeast Hsp70 cochaperones by intramolecular interactions involving their J-domains

Paolo De Los Rios, Bruno Claude Daniel Fauvet, Adélaïde Alice Mohr, Mathieu Rebeaud, Satyam Tiwari

The 70 kDa heat shock protein (Hsp70) chaperones control protein homeostasis in all ATP-containing cellular compartments. J-domain proteins (JDPs) coevolved with Hsp70s to trigger ATP hydrolysis and catalytically upload various substrate polypeptides in ne ...

Elsevier Science Inc2024

Microfluidics and cell-free synthetic biology in the development of new diagnostic and therapeutic platforms

Grégoire Michielin

In phenylketonuria, absence or malfunction of the phenylalanine hydroxylase enzyme results in toxic accumulation of phenylalanine in the body. An injectable recombinant enzyme therapy was recently approved and has the potential to improve the quality of li ...

EPFL2021

Proteasomal and lysosomal clearance of faulty secretory proteins: ER-associated degradation (ERAD) and ER-to-lysosome-associated degradation (ERLAD) pathways

Maurizio Molinari

About 40% of the eukaryotic cell's proteins are inserted co- or post-translationally in the endoplasmic reticulum (ER), where they attain the native structure under the assistance of resident molecular chaperones and folding enzymes. Subsequently, these pr ...

2019

Cytosolic delivery and characterization of monobodies interfering with SH2 domain-phosphotyrosine interactions

Nadine Eliane Schmit

Targeted cancer therapy is a very promising concept; however, small molecule inhibitors only exist for a limited number of oncoproteins and resistance development limits their clinical use. Different engineered non-antibody scaffolds are being explored to ...

EPFL2018

Cytosolic Proteostasis Networks of the Mitochondrial Stress Response

Johan Auwerx, Vincenzo Sorrentino, Davide D'Amico

Mitochondrial stress requires timely intervention to prevent mitochondria' and cellular dysfunction. Re-establishing the correct protein homeostasis is crucial for coping with mitochondria' stress and maintaining cellular homeostasis. The best-characterize ...

Elsevier Science London2017

Endosomes: Guardians Against [Ru(Phen)3]2+ Photo-action In Endothelial Cells During In Vivo pO2 Detection?

Georges Wagnières, Veronika Huntosova

Phototoxicity is a side-effect of in vitro and in vivo oxygen partial pressure (pO(2)) detection by luminescence lifetime measurement methods. Dichlorotris(1,10-phenanthroline)-ruthenium(II) hydrate (Ru(Phen)(3)) is a water soluble pO(2) probe associ ...

Royal Soc Chemistry2014

ERAD and ERAD tuning: disposal of cargo and of ERAD regulators from the mammalian ER

Maurizio Molinari

The endoplasmic reticulum (ER) is the site of maturation for secretory and membrane proteins in eukaryotic cells. Unsuccessful folding attempts are eventually interrupted and most folding-defective polypeptides are dislocated across the ER membrane and deg ...

2011

Advanced Fluorescence Microscopy to Study Plasma Membrane Protein Dynamics

Joachim Piguet

Membrane protein dynamics is of great importance for living organisms. The precise localization of proteins composing a synapse on the membrane facing a nerve terminus is essential for proper functioning of the nervous system. In muscle fibers, the nicotin ...

EPFL2010