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Concept

Mad2

Publications associées (22)

Principles of checkpoint override

Ahmad Sadeghi

Why biological quality-control systems fail is often mysterious. Specifically, checkpoints such as the DNA damage checkpoint or the spindle assembly checkpoint are overriden after prolonged arrests allowing cells to continue dividing despite the continued ...

EPFL2022

Live imaging screen reveals that TYRO3 and GAK ensure accurate spindle positioning in human cells

Pierre Gönczy, Coralie Busso, Benita Wolf

Proper spindle positioning is crucial for spatial cell division control. Spindle positioning in human cells relies on a ternary complex comprising G alpha i1-3, LGN and NuMA, which anchors dynein at the cell cortex, thus enabling pulling forces to be exert ...

Springer2019

TRACMIT: An effective pipeline for tracking and analyzing cells on micropatterns through mitosis

Pierre Gönczy, Olivier Burri, Arne Seitz, Benita Wolf

The use of micropatterns has transformed investigations of dynamic biological processes by enabling the reproducible analysis of live cells using time-lapse fluorescence microscopy. With micropatterns, thousands of individual cells can be efficiently image ...

2017

Zika virus causes supernumerary foci with centriolar proteins and impaired spindle positioning

Pierre Gönczy, Coralie Busso, Benita Wolf

Zika virus (ZIKV) causes congenital microcephaly. Although ZIKV can impair cell cycle progression and provoke apoptosis, which probably contributes to disease aetiology through depletion of neural progenitor cells, additional cellular mechanisms may be imp ...

Royal Society, The2017

Aurora A kinase regulates proper spindle positioning in C-elegans and in human cells

Pierre Gönczy, Coralie Busso, Sachin Kotak

Accurate spindle positioning is essential for error-free cell division. The one-cell Caenorhabditis elegans embryo has proven instrumental for dissecting mechanisms governing spindle positioning. Despite important progress, how the cortical forces that act ...

2016

Analysis of S. pombe SIN protein association to the SPB reveals two genetically separable states of the SIN

Michaël Unser, Daniel Sage, Viesturs Simanis, Daniel Andreas Schmitter, Andrea Krapp, Jacques Rougemont

The Schizosaccharomyces pombe septation initiation network (SIN) regulates cytokinesis, and asymmetric association of SIN proteins with the mitotic spindle pole bodies (SPBs) is important for its regulation. Here, we have used semi-automated image analysis ...

Company Of Biologists Ltd2015

NuMA interacts with phosphoinositides and links the mitotic spindle with the plasma membrane

Pierre Gönczy, Coralie Busso, Sachin Kotak

The positioning and the elongation of the mitotic spindle must be carefully regulated. In human cells, the evolutionary conserved proteins LGN/Gai1-3 anchor the coiled-coil protein NuMA and dynein to the cell cortex during metaphase, thus ensuring proper s ...

2014

Optimization of the analogue-sensitive Cdc2/Cdk1 mutant by in vivo selection eliminates physiological limitations to its use in cell cycle analysis

Viesturs Simanis

Analogue-sensitive (as) mutants of kinases are widely used to selectively inhibit a single kinase with few off-target effects. The analogue-sensitive mutant cdc2-as of fission yeast (Schizosaccharomyces pombe) is a powerful tool to study the cell cycle, bu ...

Royal Society, The2014

Characterizing the localization and role of lin-5 and era-1 mRNAs in early C. elegans embryos

Zoltán Péter Spiró

Asymmetric cell division is essential for the generation of diversity during development and the function of stem cell lineages. The Caenorhabditis elegans zygote is an attractive model to investigate the mechanisms of spindle positioning during asymmetric ...

EPFL2014

Clathrin regulates centrosome positioning by promoting acto-myosin cortical tension in C. elegans embryos

Pierre Gönczy, Alessandro De Simone, Sylvain Träger, Kalyani Thyagarajan

Regulation of centrosome and spindle positioning is crucial for spatial cell division control. The one-cell Caenorhabditis elegans embryo has proven attractive for dissecting the mechanisms underlying centrosome and spindle positioning in a metazoan organi ...

Company Of Biologists Ltd2014

Mechanisms of spindle positioning: cortical force generators in the limelight

Pierre Gönczy, Sachin Kotak

Correct positioning of the spindle governs placement of the cytokinesis furrow and thus plays a crucial role in the partitioning of fate determinants and the disposition of daughter cells in a tissue. Converging evidence indicates that spindle positioning ...

2013

Cortical dynein is critical for proper spindle positioning in human cells

Pierre Gönczy, Coralie Busso, Sachin Kotak

Correct spindle positioning is fundamental for proper cell division during development and in stem cell lineages. Dynein and an evolutionarily conserved ternary complex (nuclear mitotic apparatus protein [NuMA]–LGN–Gα in human cells and LIN-5–GPR-1/2–Gα in ...

Rockefeller University Press2012

Towards understanding HsSAS-6, a key player in centriole duplication

Debora Keller

The centrosome is the cell’s main microtubule organizing center. In order to assemble a proper bipolar spindle in mitosis, the cell has to duplicate its inherited single centrosome. During centrosome duplication, a single procentriole assembles approximate ...

EPFL2012

Mutual Inhibition Between the Anaphase-promoting Complex and the Spindle Assembly Checkpoint in C. Elegans Embryos

Alexandra Bezler

The cell cycle orchestrates timely duplication and distribution of cellular components to daughter cells. Checkpoints operate to survey the faithful completion of specific steps during the cell cycle, thus ensuring the equal segregation of genetic material ...

EPFL2011

Asymmetric Spindle Positioning and Intracellular Trafficking in C. Elegans Embryos

Kalyani Thyagarajan

Asymmetric cell division is critical for generating daughter cells that possess distinct fates during development and in stem cell lineages. Correct orientation of the mitotic spindle with respect to the polarity axis is crucial to this process. In the one ...

EPFL2011

Regulation of cortical contractility and spindle positioning by the protein phosphatase 6 PPH-6 in one-cell stage C. elegans embryos

Pierre Gönczy

Modulation of the microtubule and the actin cytoskeleton is crucial for proper cell division. Protein phosphorylation is known to be an important regulatory mechanism modulating these cytoskeletal networks. By contrast, there is a relative paucity of infor ...

2010

Mutual Antagonism Between the Anaphase Promoting Complex and the Spindle Assembly Checkpoint Contributes to Mitotic Timing in Caenorhabditis elegans

Pierre Gönczy, Alexandra Bezler

The anaphase promoting complex/cyclosome (APC/C) triggers the separation of sister chromatids and exit from mitosis across eukaryotic evolution. The APC/C is inhibited by the spindle assembly checkpoint (SAC) until all chromosomes have achieved bipolar att ...

2010

Phosphorylation state defines discrete roles for monopolin in chromosome attachment and spindle elongation

Viesturs Simanis

BACKGROUND: It is unknown how oscillations in Cdk1 activity drive the dramatic changes in chromosome and spindle dynamics that occur at the metaphase/anaphase transition. RESULTS: We show that the Schizosaccharomyces pombe monopolin complex has distinct fu ...

2009

Lte1, Cdc14 and MEN-controlled Cdk inactivation in yeast coordinate rDNA decompaction with late telophase progression

Thierry Laroche

The mechanism of chromatin compaction in mitosis has been well studied, while little is known about what controls chromatin decompaction in early G1 phase. We have localized the Condensin subunit Brn1 to a compact spiral of rDNA in mitotic budding yeast ce ...

Nature Publishing Group2009

c-Myc and its target FoxM1 are critical downstream effectors of constitutive androstane receptor (CAR) mediated direct liver hyperplasia

Sandra Eloise Kjeldsen, Christelle Dubey

In the adult liver, 1,4-bis[2-(3,5-dichloropyridyloxy)] benzene (TCPOBOP), an agonist of the constitutive androstane receptor (CAR, NR1I3), produces rapid hepatomegaly in the absence of injury. In this study, we identify c-Myc as a gene induced by CAR and ...

2008