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Concept

Sélection clonale

Publications associées (18)

Microfluidic T Cell Selection by Cellular Avidity

Li Tang, Alexandre Harari, Armand Kurum

No T cell receptor (TCR) T cell therapies have obtained clinical approval. The lack of strategies capable of selecting and recovering potent T cell candidates may be a contributor to this. Existing protocols for selecting TCR T cell clones for cell therapi ...

WILEY2022

Label-free identification of activated T lymphocytes through tridimensional microsensors on chip

Carlotta Guiducci, George Coukos, Alexandre Harari, Elena Bianchi, Enrico Tenaglia, Enrica Rollo

Label-free approaches to assess cell properties ideally suit the requirements of cell-based therapeutics, since they permit to characterize cells with minimal perturbation and manipulation, at the benefit of sample recovery and re-employment for treatment. ...

Elsevier2017

Encapsulation of Insulin-Secreting Cells Expressing a Genetically Encoded Fluorescent Calcium Indicator for Cell-Based Sensing In Vivo

Nicolas Bouche, Andreas Wiederkehr, Christophe Boss

The development of cell-based biosensors that give insight into cell and tissue function in vivo is an attractive technology for biomedical research. Here, the development of a cell line expressing a fluorescent calcium sensor for the study of beta-cell fu ...

Wiley2017

Exploring the potency of normal and pathological human thymic epithelial cells

Tiphaine Mélodie Chantal Arlabosse

Thymic epithelial cells (TECs) are organised in a unique 3D network that is critical for the development of efficient and self-tolerant T-cells. We report for the first time that the human thymus of all ages, even old and involuted, contains a significant ...

EPFL2017

Phage selection of chemically stabilized alpha-helical peptide ligands

Christian Heinis, Jörg Hülsken, Davide Bertoldo, Pierre Vincent Dessen, Philippe Diderich, Maola Khan Mohammad Golam

Short alpha-helical peptides stabilized by linkages between constituent amino acids offer an attractive format for ligand development. In recent years, a range of excellent ligands based on stabilized alpha-helices were generated by rational design using a ...

2016

Screening of structurally diverse bicyclic peptide libraries by phage display

Davide Bertoldo

Bicyclic peptides are an attractive molecule format for the development of therapeutics and research tools. Our laboratory is specialized on the development of bicyclic peptide ligands by phage display. In brief, libraries of randompeptides each containing ...

EPFL2016

Protein engineering approaches for direct antigen targeting in CD8+ T cell inducing vaccines

Vasiliki Panagiotou

Effective vaccine design and public vaccination programs have led to the eradication of several diseases and protected millions of people from deadly infections. However, intracellular infections remain a challenge and their eradication requires the activa ...

EPFL2016

Cell motion predicts human epidermal stemness

Yann Barrandon

Image-based identification of cultured stem cells and noninvasive evaluation of their proliferative capacity advance cell therapy and stem cell research. Here we demonstrate that human keratinocyte stem cells can be identified in situ by analyzing cell mot ...

Rockefeller University Press2015

Immune Antibodies and Helminth Products Drive CXCR2-Dependent Macrophage-Myofibroblast Crosstalk to Promote Intestinal Repair

Nicola Harris, Beatrice Volpe, Julia Esser-von Bieren, Duncan-Bruce Sutherland, Jérôme Bürgi

Helminth parasites can cause considerable damage when migrating through host tissues, thus making rapid tissue repair imperative to prevent bleeding and bacterial dissemination particularly during enteric infection. However, how protective type 2 responses ...

Public Library Science2015

Clonal, Self-Renewing and Differentiating Human and Porcine Urothelial Cells, a Novel Stem Cell Population

Jeffrey Alan Hubbell, Peter Frey, Yann Barrandon, Hans Mattias Larsson, François Gorostidi

Although urothelial progenitor-like cells have been described in the human urinary tract, the existence of stem cells remains to be proven. Using a culture system that favors clonogenic epithelial cell growth, we evaluated and characterized clonal human ur ...

Public Library of Science2014

CHO Quasispecies—Implications for Manufacturing Processes

Florian Maria Wurm

Chinese hamster ovary (CHO) cells are a source of multi-ton quantities of protein pharmaceuticals. They are, however, immortalized cells, characterized by a high degree of genetic and phenotypic diversity. As is known for any biological system, this divers ...

MDPI2013

Phage display libraries of differently sized bicyclic peptides

Christian Heinis, Alessandro Angelini, Inmaculada Rentero Rebollo

Phage selections with combinatorial libraries of uniformly sized bicyclic peptides have recently yielded potent and selective binders of several protein targets. In this work we varied in a combinatorial fashion the ring sizes of bicyclic peptides in phage ...

Royal Soc Chemistry2013

Mathematical Modeling of T-Cell Experimental Data

Irina Baltcheva

T lymphocytes (T cells) are key components of the adaptive immune system. These cells are able to recognize an enormous variety of pathogens thanks to the great specificity of their trans-membrane proteins, the T cell receptors (TCRs). TCR diversity is cre ...

EPFL2010

FcRgamma activation regulates inflammation-associated squamous carcinogenesis

Michele De Palma

Chronically activated leukocytes recruited to premalignant tissues functionally contribute to cancer development; however, mechanisms underlying pro- versus anti-tumor programming of neoplastic tissues by immune cells remain obscure. Using the K14-HPV16 mo ...

2010

Heterogeneity in multistage carcinogenesis and mixture modeling

Sandro Gsteiger

Carcinogenesis is commonly described as a multistage process. In a first step, a stem cell is transformed via a series of mutations into an intermediate cell having a growth advantage. Under favorable conditions, such a cell will give rise to a clone of in ...

EPFL2006

Inducing tolerance to a soluble foreign antigen by encapsulated cell transplants

Patrick Aebischer, Bernard Schneider

The immune response to soluble antigens constitutes a current clinical problem impeding the development of protein therapeutics. We have developed an encapsulated-cell delivery system, which, transiently combined with an anti-CD154 antibody treatment, allo ...

2006

Dendritic-cell trafficking to lymph nodes through lymphatic vessels

Melody Swartz

Antigen-presenting dendritic cells often acquire foreign antigens in peripheral tissues such as the skin. Optimal encounter with naive T cells for the presentation of these antigens requires that the dendritic cells migrate to draining lymph nodes through ...

2005

Central nervous system delivery of recombinant ciliary neurotrophic factor by polymer encapsulated differentiated C2C12 myoblasts

Patrick Aebischer, Nicole Déglon

Neurotrophic factors hold promise for the treatment of neurodegenerative diseases. Intrathecal transplantation of polymer encapsulated cell lines genetically engineered to release neurotrophic factors provides a means to deliver them continuously behind th ...

1996