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Publications associées (27)

Page 1 sur 2

Development of Standardized Fetal Progenitor Cell Therapy for Cartilage Regenerative Medicine: Industrial Transposition and Preliminary Safety in Xenogeneic Transplantation

Dominique Pioletti, Corinne Scaletta, Sandra Jaccoud, Philippe Abdel Sayed, Nathalie Hirt-Burri, Salim Elias Darwiche

Diverse cell therapy approaches constitute prime developmental prospects for managing acute or degenerative cartilaginous tissue affections, synergistically complementing specific surgical solutions. Bone marrow stimulation (i.e., microfracture) remains a ...

2021

Proof-of-concept analytical instrument for label-free optical deconvolution of protein species in a mixture

Gabriel Aeppli

The adoption of process analytical technologies by the biopharmaceutical industry can reduce the cost of therapeutic drugs and facilitate investigation of new bioprocesses. Control of critical process parameters to retain critical product quality attribute ...

ELSEVIER2021

Naming CHO cells for bio-manufacturing: Genome plasticity and variant phenotypes of cell populations in bioreactors question the relevance of old names

Florian Maria Wurm

Chinese Hamster Ovary [CHO] cells are the workhorse for production of modern biopharmaceuticals. They are however immortalized cells with a high propensity for genetic change. Judging from published culture records, CHO cell populations have undergone hund ...

2021

Therapeutic approaches against coronaviruses acute respiratory syndrome

Francesco Stellacci

Coronaviruses represent global health threat. In this century, they have already caused two epidemics and one serious pandemic. Although, at present, there are no approved drugs and therapies for the treatment and prevention of human coronaviruses, several ...

JOHN WILEY & SONS LTD2021

Considerations of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection-A Simulation Study

Florian Maria Wurm

For the manufacturing of complex biopharmaceuticals using bioreactors with cultivated mammalian cells, high product concentration is an important objective. The phenotype of the cells in a reactor plays an important role. Are clonal cell populations showin ...

2021

Analyzing glycans cleaved from a biotherapeutic protein using ultrahigh-resolution ion mobility spectrometry together with cryogenic ion spectroscopy

Thomas Rizzo, Priyanka Bansal, Ali H Abikhodr, Vasyl Yatsyna, Natalia Yalovenko

Glycans covalently attached to protein biotherapeutics have a significant impact on their biological activity, clearance, and safety. As a result, glycosylation is categorized as a critical quality attribute that needs an adequate analytical approach to gu ...

2020

Active biomaterials for mechanobiology

Mahmut Selman Sakar, Berna Ayse Özkale Edelmann

Active biomaterials offer novel approaches to study mechanotransduction in mammalian cells. These material systems probe cellular responses by dynamically modulating their resistance to endogenous forces or applying exogenous forces on cells in a temporall ...

2020

The Investigation of Flory-Huggins Interaction Parameters for Amorphous Solid Dispersion Across the Entire Temperature and Composition Range

Esther Amstad

Amorphous solid dispersion (ASD) is one of the most promising enabling formulations featuring significant water solubility and bioavailability enhancements for biopharmaceutical classification system (BCS) class II and IV drugs. An accurate thermodynamic u ...

2019

Rapid Production of Submicron Drug Substance Particles by Supersonic Spray Drying

Esther Amstad

Many newly developed active pharmaceutical ingredients (APIs) are poorly soluble in water and thus have a dissolution-limited bioavailability. The bioavailability of Biopharmaceutical Classification System (BCS) class II APIs increases if they dissolve fas ...

Amer Chemical Soc2017

Therapeutic Proteins Engineered for Super-Affinity to the Extracellular Matrix in Regenerative Medicine

Priscilla Suhasna Maithili Briquez

Regenerative medicine aims to repair body tissues when natural mechanisms fail. Growth factors (GFs) are essential proteins that promote tissue regeneration upon injury, and as such, are very promising therapeutic drugs in regenerative medicine. Due to the ...

EPFL2016

The Engineering of a Scalable Disposable Orbitally Shaken Bioreactor System for Animal Cell Cultivation and Therapeutic Protein Production

Dominique Terry Monteil Jr

The aim of the pharmaceutical industry is to manufacture complex therapeutic proteins or biologics to treat human diseases. The manufacture of biologics requires a significant amount of financial and infrastructure resources. For this reason, efforts are b ...

EPFL2014

Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells

Florian Maria Wurm, Lucia Baldi Unser, Yashas Rajendra

The optimization of cell culture conditions for growth and productivity of recombinant Chinese hamster ovary (CHO) cells is a critical step in biopharmaceutical manufacturing. In the present study, the effects of the timing and amount of peptone feeding of ...

2014

A potent and selective Sirtuin 1 inhibitor alleviates pathology in multiple animal and cell models of Huntington's disease

Protein acetylation, which is central to transcriptional control as well as other cellular processes, is disrupted in Huntington's disease (HD). Treatments that restore global acetylation levels, such as inhibiting histone deacetylases (HDACs), are effecti ...

Oxford University Press2014

Personalized Drug Administrations Using Support Vector Machine

Giovanni De Micheli, Alena Simalatsar, Wenqi You Dubout

The decision-making process regarding drug dose, regularly used in everyday medical practice, is critical to patients’ health and recovery. It is a challenging process, especially for a drug with narrow therapeutic ranges, in which a medical doctor decides ...

2013

Cell biology: a key driver of therapeutic innovation

Oliver Hantschel

All processes associated with cellular function are likely to contribute to disease. Particularly in the cancer field, most major therapeutic innovations have originated from the elucidation of basic molecular mechanisms by academic researchers. Recent bre ...

Rockefeller University Press2012

Capturing epidermal stemness for regenerative medicine

Yann Barrandon, Ariane Rochat, François Gorostidi, Stéphanie Claudinot, Andrea Zaffalon, Stéphanie Danièle Hélène Droz-Georget, Nicolas Grasset

The skin is privileged because several skin-derived stem cells (epithelial stem cells from epidermis and its appendages, mesenchymal stem cells from dermis and subcutis, melanocyte stem cells) can be efficiently captured for therapeutic use. Main indicatio ...

Academic Press Ltd- Elsevier Science Ltd2012

Drug development: longer-lived proteins

Jeffrey Alan Hubbell

Protein therapeutics represent a powerful class of clinically approved drugs for the prevention and treatment of various diseases. Once administered, the biological fate of protein therapeutics is governed by the body's various complex biochemical and biop ...

Royal Society of Chemistry2012

Manufacturing recombinant proteins in kg-ton quantities using animal cells in bioreactors

Florian Maria Wurm

Mammalian cells in bioreactors as production host are the focus of this review. We wish to briefly describe today's technical status and to highlight emerging trends in the manufacture of recombinant therapeutic proteins, focusing on Chinese hamster ovary ...

Elsevier2011

OrbShake Bioreactors for Mammalian Cell Cultures : Engineering and Scale-up

Most of the biopharmaceuticals nowadays are produced by animal cells. The worldwide demand for a specific group of biopharmaceuticals, the monoclonal antibodies (mAbs), has significantly increased this last decade. The mAbs are used as therapeutics in part ...

EPFL2011

Transient Gene Expression in HEK-293E Cells for Recombinant Protein Production : Process Characterization towards GMP Approval

The growing demand of biopharmaceutical products is boosting the market for therapeutic recombinant proteins (r-proteins). More than half of the 140 r-proteins that have gained approval for human therapeutic use are manufactured in mammalian cells that mak ...

EPFL2010