Concept

Fluorescence in the life sciences

Publications associées (31)

Photoactivatable Silicon Rhodamines for Super-Resolution Microscopy

Fluorescence microscopy is the method of choice to monitor dynamic processes in living cells due to its non-invasive nature. A variety of different fluorophores and labeling systems are currently used to selectively visualise structures or biomolecules of ...

EPFL2020

Promising anti-cancer potency of 4,5,6,7-tetrahydrobenzo[d]thiazole-based Schiff-bases

Kurt Schenk, Farzaneh Fadaei Tirani

Four new Schiff-bases have been synthesized from the 1:2 M condensation of 4,5,6,7-tetrahydrobenzothiazole-2,6-diamine with 2-hydroxybenzaldehyde (SL3), 5-Bromo-2-hydroxybenzaldehyde (SL2), 2-hydroxy-3-methoxybenzaldehyde (SL3) and 2-hydroxy-1-naphthaldehy ...

ELSEVIER2020

"Doubly Orthogonal" Labeling of Peptides and Proteins

Jérôme Waser, Beat Fierz, Nora Guidotti, Raphaël Michel Henri Simonet-Davin, Romain Gaëtan Tessier, Javier De Ceballos Cerrajeria

Herein, we report a cysteine bioconjugation methodology for the introduction of hypervalent iodine compounds onto biomolecules. Ethynylbenziodoxolones (EBXs) engage thiols in small organic molecules and cysteine-containing peptides and proteins in a fast a ...

2019

Mechanisms of fluorescence quenching in prototypical aggregation-induced emission systems: excited state dynamics with TD-DFTB

Anne-Clémence Corminboeuf, Antonio Prlj, Kun-Han Lin, Daniel Hollas

A recent implementation of time-dependent tight-binding density functional theory is employed in excited state molecular dynamics for the investigation of the fluorescence quenching mechanism in 3 prototypical aggregation-induced emission systems. An asses ...

ROYAL SOC CHEMISTRY2019

Digital staining through the application of deep neural networks to multi-modal multi-photon microscopy

Demetri Psaltis, Navid Borhani

Deep neural networks have been used to map multi-modal, multi-photon microscopy measurements of a label-free tissue sample to its corresponding histologically stained brightfield microscope colour image. It is shown that the extra structural and functional ...

2019

Fluorescence lifetime imaging with a single-photon SPAD array using long overlapping gates: an experimental and theoretical study

Edoardo Charbon, Claudio Bruschini, Arin Can Ülkü, Andrei Ardelean

Developing large arrays of single-photon avalanche diodes (SPADs) with on-chip time-correlated single-photon counting (TCSPC) capabilities continues to be a difficult task due to stringent silicon real estate constraints, high data rates and system complex ...

2019

Preexisting variation in DNA damage response predicts the fate of single mycobacteria under stress

John McKinney, Neeraj Dhar, Giulia Manina

Clonal microbial populations are inherently heterogeneous, and this diversification is often considered as an adaptation strategy. In clinical infections, phenotypic diversity is found to be associated with drug tolerance, which in turn could evolve into g ...

2019

Nanoscale Lithography and Thermometry with Thermal Scanning Probes

Samuel Tobias Howell

Thermal scanning probe lithography (t-SPL) is an advanced lithography technique in which a heated atomic force microscope tip locally modifies a sample material. Due to the nanometer-sized tip apex diameter, t-SPL enables patterning of sub-10 nm structures ...

EPFL2018

Applications of a reconfigurable SPAD line imager

Edoardo Charbon, Claudio Bruschini, Samuel Burri

Single-photon avalanche diodes (SPADs) fabricated in standard CMOS processes enable cost-effective volume production of sensors capable of time-stamping individual photons with high accuracy. This capability makes the sensors ideally suited for application ...

2018

Synthesis and Postpolymerization Modification of Fluorine-End-Labeled Poly(Pentafluorophenyl Methacrylate) Obtained via RAFT Polymerization

Harm-Anton Klok, Claudia Battistella

Chain-end-labeled polymers are interesting for a range of applications. In polymer nanomedicine, chain-end-labeled polymers are useful to study and help understand cellular internalization and intracellular trafficking processes. The recent advent of fluor ...

2018

A time-gated large-array SPAD camera for picosecond resolution real-time FLIM

Edoardo Charbon, Claudio Bruschini, Arin Can Ülkü

Single-photon avalanche diode (SPAD) arrays have recently emerged as promising detectors for many wide-field fluorescence lifetime imaging microscopy (FLIM) applications, thanks to their picosecond range temporal resolution, single-photon sensitivity and f ...

2018