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Concept

Selected reaction monitoring

Publications associées (23)

Electron spectrometer calibration method

Tobias Kippenberg, Fabrizio Carbone, Arslan Sajid Raja, Thomas La Grange, Yujia Yang, Alexey Sapozhnik, Bruce Michael Raleigh Weaver, Paolo Cattaneo

The present invention concerns an energy dispersion calibration method for calibrating an electron spectrometer of an electron spectrometer system including at least one electron emission source, electron optics and the electron spectrometer. The method co ...

2023

Biological nanopores for single-molecule sensing

Matteo Dal Peraro, Chan Cao, Simon Finn Mayer

Evolution has found countless ways to transport material across cells and cellular compartments separated by membranes. Protein assemblies are the cornerstone for the formation of channels and pores that enable this regulated passage of molecules in and ou ...

Cell Press2022

Ultraslow radiative cooling of Cn- (n=3-5)

Eduardo Carrascosa Casado

Ultraslow radiative cooling lifetimes and adiabatic detachment energies for three astrochemically relevant anions, Cn- (n = 3-5), are measured using the Double ElectroStatic Ion Ring ExpEriment (DESIREE) infrastructure at Stockholm University. DESIREE main ...

AMER INST PHYSICS2019

Transition metal clusters on h-BN/Rh(111) : surface interaction and catalytic activity

Hamed Achour

The main aim of this dissertation is to study the catalytic aspects of very small transition metal clusters supported on h-BN/Rh(111). The catalytic reactions of interest were ammonia synthesis and CO oxidation on mass selected and soft landed iron and pla ...

EPFL2017

Identification of hemoglobin variants by top-down mass spectrometry using selected diagnostic product ions

Yury Tsybin

Hemoglobin disorder diagnosis is a complex procedure combining several analytical steps. Due to the lack of specificity of the currently used protein analysis methods, the identification of uncommon hemoglobin variants (proteoforms) can become a hard task ...

Springer Heidelberg2015

On the use of electron capture rate constants to describe electron capture dissociation mass spectrometry of peptides

Yury Tsybin, Konstantin Zhurov, Aleksey Vorobyev, Unige Anna Laskay

Electron capture dissociation (ECD) tandem mass spectrometry (MS/MS) is a powerful analytical tool for peptide and protein structure analysis. The product ion abundance (PIA) distribution in ECD MS/MS is known to vary as a function of electron irradiation ...

Im Publications2015

From allergen detection to protein-ligand interactions : novel mass spectrometry-based analytical methods

Natalia Gasilova

Fundamental biological and biochemical studies, proteomics, practical nutrition-related and medical applications, all the research fields, where bioanalytical chemistry is an essential tool, constantly require the improvement of existing techniques of bioa ...

EPFL2014

Electron Transfer Dissociation Mass Spectrometry of Hemoglobin on Clinical Samples

Yury Tsybin

A mass spectrometry-based assay combining the specificity of selected reaction monitoring and the protein ion activation capabilities of electron transfer dissociation was developed and employed for the rapid identification of hemoglobin variants from whol ...

Springer2012

Inactivation of bacteriophage MS2 with potassium ferrate(VI)

Tamar Kohn, Anne Thérèse Sigstam, Timothy Strathmann

Ferrate [Fe(VI); FeO42-] is an emerging oxidizing agent capable of controlling chemical and microbial water contaminants. Here, inactivation of MS2 coliphage by Fe(VI) was examined. The inactivation kinetics observed in individual batch experiments was wel ...

Amer Chemical Soc2012

On the utility of predictive chromatography to complement mass spectrometry based intact protein identification

Yury Tsybin, Hisham Ben Hamidane

The amino acid sequence determines the individual protein three-dimensional structure and its functioning in an organism. Therefore, "reading" a protein sequence and determining its changes due to mutations or post-translational modifications is one of the ...

Springer-Verlag2012

Combination of Gas-Phase Fractionation and MS3 Acquisition Modes for Relative Protein Quantification with Isobaric Tagging

Loïc Dayon

Relative quantification of peptides and proteins with isobaric tags such as iTRAQ or TMT is commonly used in comparative quantitative proteomics based on tandem mass spectrometry (MS/MS). Nonetheless, isobaric tagging inherently suffers from the cofragment ...

Amer Chemical Soc2012

Repeatability and reproducibility of product ion abundances in electron capture dissociation mass spectrometry of peptides

Yury Tsybin, Aleksey Vorobyev, Hisham Ben Hamidane

Site-specific reproducibility and repeatability of electron capture dissociation (ECD) in Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) are of fundamental importance for product ion abundance (PIA)-based peptide and protein struct ...

2011

Peptide Structure and Radical Chemistry in the Gas Phase Revealed by Product Ion Abundance Analysis in Electron Capture and Transfer Dissociation Mass Spectrometry

Hisham Ben Hamidane

Peptide and protein ion abundances in mass spectrometry (MS) and tandem mass spectrometry (MS/MS) may provide orthogonal, or complementary, information to their mass measurements. Collision induced dissociation (CID), the most commonly used MS/MS technique ...

EPFL2011

Incremental Identification of Reaction and Mass-Transfer Kinetics Using the Concept of Extents

Dominique Bonvin, Michael Amrhein, Nirav Pravinbhai Bhatt

This paper proposes a variation of the incremental approach to identify reaction and mass-transfer kinetics (rate expressions and the corresponding rate parameters) from concentration measurements for both homogeneous and gas-liquid reaction systems. This ...

2011

Spectroscopy and dissociation dynamics of cold, biomolecular ions

The function of a peptide is intimately connected to its structure, which in solution is the result of the balance between non-covalent interactions within the molecule and the molecule-solvent interactions. Theory has the power to predict the properties o ...

EPFL2010

Photocatalytic Redox Reactions for In-Source Peptide Fragmentation

Hubert Girault, Jérôme Waser, Liang Qiao, Hongyan Bi

In-source photocatalytic redox reactions based on a photosensitive target plate have been developed to realize peptide fragmentation during laser desorption ionization. Sample peptides and glucose are simply deposited on a spot of sintered TiO2 nanoparticl ...

2009

Periodic Sequence Distribution of Product Ion Abundances in Electron Capture Dissociation of Amphipathic Peptides and Proteins

Yury Tsybin, Hisham Ben Hamidane

The rules for product ion formation in electron capture dissociation (ECD) mass spectrometry of peptides and proteins remain unclear. Random backbone cleavage probability and the nonspecific nature of ECD toward amino acid sequence have been reported, cont ...

Springer-Verlag2009

Electron Capture and Transfer Dissociation: Peptide Structure Analysis at Different Ion Internal Energy Levels

Yury Tsybin, Marc Moniatte, Aleksey Vorobyev, Diego Chiappe, Hisham Ben Hamidane

We decoupled electron-transfer dissociation (ETD) and collision-induced dissociation of charge-reduced species (CRCID) events to probe the lifetimes of intermediate radical species in ETD-based ion trap tandem mass spectrometry of peptides. Short-lived int ...

Springer-Verlag2009

Exploring metallodrug-protein interactions by mass spectrometry: comparisons between platinum coordination complexes and an organometallic ruthenium compound

Paul Joseph Dyson, Angela Casini

Electrospray ionisation mass spectrometry was used to analyze the reactions of metal compds. with mixts. of selected proteins. Three representative medicinally relevant compds., cisplatin, transplatin and the organometallic ruthenium compd. RAPTA-C, were r ...

Springer2009

Electrospray ion beam deposition and mass spectrometry of nonvolatile molecules and nanomaterials

Stephan Rauschenbach

The vacuum deposition of complex functional molecules and nanoparticles by thermal sublimation is often hindered due to their extremely low vapor pressure. This especially impedes the application of ultrahigh vacuum (UHV) based analytical and surface modif ...

EPFL2008