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Publications associées (31)

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Unraveling the KRAB/KAP1 control of transposable elements in pluripotent and somatic cells

Gabriela Ecco

Transposable elements (TEs) are DNA sequences able to change position in the genome, and represent more than 40% of mammalian genetic material. TEs can have positive or detrimental effects on the host, being both important motors of evolution and genomic t ...

EPFL2016

Microfluidic encapsulation of cells in alginate particles via an improved internal gelation approach

Samin Akbari

An improved internal gelation approach is developed to encapsulate single mammalian cells in monodisperse alginate microbeads as small as 26 mu m in diameter and at rates of up to 1 kHz with high cell viability. The cell damage resulting from contact with ...

Springer Heidelberg2014

Analysis of centriole elimination during C. elegans oogenesis

Graham Knott, Pierre Gönczy, Petr Strnad, Lukas von Tobel, Tamara Mikeladze-Dvali

Centrosomes are the principal microtubule organizing centers (MTOCs) of animal cells and comprise a pair of centrioles surrounded by pericentriolar material (PCM). Centriole number must be carefully regulated, notably to ensure bipolar spindle formation an ...

2012

Isolation and Characterisation of Regulators of the Septation Initiation Network Regulators in Fission Yeast

Anupama Goyal

The septation initiation network (SIN) in Schizosaccharomyces pombe regulates cytokinesis. Cdc7p encodes the first kinase in the core SIN pathway. In this study, I performed a genetic screen to identify novel regulators of the SIN pathway by isolating spon ...

EPFL2012

Malaria parasites form filamentous cell-to-cell connections during reproduction in the mosquito midgut

Physical contact is important for the interaction between animal cells, but it can represent a major challenge for protists like malaria parasites. Recently, novel filamentous cell-cell contacts have been identified in different types of eukaryotic cells a ...

2011

Epigenetic memory in induced pluripotent stem cells

Olaia Maria Naveiras Torres-Quiroga, Kyungjin Kim

Somatic cell nuclear transfer and transcription-factor-based reprogramming revert adult cells to an embryonic state, and yield pluripotent stem cells that can generate all tissues. Through different mechanisms and kinetics, these two reprogramming methods ...

2010

U(VI) reduction by spores of Clostridium acetobutylicum

Rizlan Bernier-Latmani, Elena Suvorova Buffat, Harish Veeramani, Elena Cristina Dalla Vecchia

Vegetative cells of Clostridium acetobutylicum are known to reduce hexavalent uranium (U(VI)). We investigated the ability of spores of this organism to drive the same reaction. We found that spores were able to remove U(VI) from solution when H, was provi ...

Elsevier2010

In Drosophila melanogaster the COM Locus Directs the Somatic Silencing of Two Retrotransposons through both Piwi-Dependent and -Independent Pathways

Background. In the Drosophila germ line, repeat-associated small interfering RNAs (rasiRNAs) ensure genomic stability by silencing endogenous transposable elements. This RNA silencing involves small RNAs of 26-30 nucleotides that are mainly produced from t ...

Public Library of Science2008

Mechanisms of Disease: cancer stem cells - targeting the evil twin

Classical antineoplastic treatments such as chemotherapy or radiation can efficiently eradicate the majority of proliferating and genetically unstable malignant cells within neoplastic lesions. There is increasing evidence, however, that these regimens fre ...

2008

Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

Florian Maria Wurm, Gaurav Backliwal, Sébastien Chenuet, Sarah Wulhfard

Transient transfection allows for fast production of recombinant proteins. However, the current bottlenecks in transient transfection are low titers and low specific productivity compared to stable cell lines. Here, we report an improved transient transfec ...

2008

Stem cells and their niche: a matter of fate

Olaia Maria Naveiras Torres-Quiroga

Embryonic stem cells provide an in vitro model for developmental biologists to study cell fate decisions during ontogenesis, while somatic stem cells allow physiologists to understand tissue homeostasis in the adult. The behavior of stem cells is dependent ...

Springer Verlag2006