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Concept

PLK1

Publications associées (18)

Centromere structure and function: lessons from Drosophila

Eftychia Kyriacou

The fruit fly Drosophila melanogaster serves as a powerful model organism for advancing our understanding of biological processes, not just by studying its similarities with other organisms including ourselves but also by investigating its differences to u ...

Genetics Society America2023

Dissecting centriole fate in C. elegans development

Nils Kalbfuss

The presence of centrioles is critical for fundamental cell and developmental processes, including polarity, migration and division. Although centrioles are present in most proliferating cells, they are thought to vanish after cell cycle exit in some diffe ...

EPFL2022

Centriole foci persist in starfish oocytes despite Polo-like kinase 1 inactivation or loss of microtubule nucleation activity

Pierre Gönczy, Nils Kalbfuss, Marie Juliette Ségolène Pierron

Centrioles must be eliminated or inactivated from the oocyte to ensure that only the two functional centrioles contributed by the sperm are present in the zygote. Such removal can occur during oogenesis, as in Drosophila, where departure of Polo kinase fro ...

2020

Fluorogenic probes for live-cell imaging

Aleksandar Salim

The development of novel fluorogenic probes and their use in live-cell imaging lead to a plethora of discoveries in biological research. Herein I report on novel red fluorogenic probes for live-cell super-resolution microscopy of various cellular organelle ...

EPFL2020

Zika virus causes supernumerary foci with centriolar proteins and impaired spindle positioning

Pierre Gönczy, Coralie Busso, Benita Wolf

Zika virus (ZIKV) causes congenital microcephaly. Although ZIKV can impair cell cycle progression and provoke apoptosis, which probably contributes to disease aetiology through depletion of neural progenitor cells, additional cellular mechanisms may be imp ...

Royal Society, The2017

Genetic depletion of Polo-like kinase 1 leads to embryonic lethality due to mitotic aberrancies

Polo-like kinase 1 (PLK1) is a serine/threonine kinase that plays multiple and essential roles during the cell division cycle. Its inhibition in cultured cells leads to severe mitotic aberrancies and cell death. Whereas previous reports suggested that Plk1 ...

Wiley-Blackwell2016

Mechanisms of centrosome separation in C. elegans

Alessandro De Simone

Centrosomes are the major microtubule organizing centers of animal cells. The two centrosomes present at the onset of mitosis must separate in a timely fashion along the nuclear envelope to ensure proper bipolar spindle assembly and thus genome stability. ...

EPFL2016

Dynein Transmits Polarized Actomyosin Cortical Flows to Promote Centrosome Separation

Pierre Gönczy, Alessandro De Simone

The two centrosomes present at the onset of mitosis must separate in a timely and accurate fashion to ensure proper bipolar spindle assembly. The minus-end-directed motor dynein plays a pivotal role in centrosome separation, but the underlying mechanisms r ...

Elsevier2016

Purification of centrosomes from mammalian cell lines

Paul Guichard

Centrosomes act as the main microtubule-organizing centre of animal cells and play critical roles in the cell, such as mitotic spindle organization, cell polarity, and motility. They are composed of two barrel-shaped structures, the centrioles, surrounded ...

Elsevier Academic Press Inc2015

Dma1-dependent degradation of SIN proteins during meiosis in Schizosaccharomyces pombe

Viesturs Simanis, Andrea Krapp

The Schizosaccharomyces pombe septation initiation network (SIN) is required for cytokinesis during vegetative growth and for spore formation during meiosis. Regulation of the SIN during mitosis has been studied extensively, but less is known about its mei ...

Company of Biologists2014

Selective Chemical Crosslinking Reveals a Cep57-Cep63-Cep152 Centrosomal Complex

Kai Johnsson, Pierre Gönczy, Luc Reymond, Grazvydas Lukinavicius

The centrosome functions as the main microtubule-organizing center of animal cells and is crucial for several fundamental cellular processes [1]. Abnormalities in centrosome number and composition correlate with tumor progression [2, 3] and other diseases ...

Elsevier2013

The SCF-FBXW5 E3-ubiquitin ligase is regulated by PLK4 and targets HsSAS-6 to control centrosome duplication

Pierre Gönczy, Debora Keller

Deregulated centrosome duplication can result in genetic instability and contribute to tumorigenesis(1,2). Here, we show that centrosome duplication is regulated by the activity of an E3-ubiquitin ligase that employs the F-box protein FBXW5 (ref. 3) as its ...

Nature Publishing Group2011

PP2A Phosphatase Acts upon SAS-5 to Ensure Centriole Formation in C. elegans Embryos

Pierre Gönczy, Debora Keller

Centrosome duplication occurs once per cell cycle and ensures that the two resulting centrosomes assemble a bipolar mitotic spindle. Centriole formation is fundamental for centrosome duplication. In Caenorhabditis elegans, the evolutionarily conserved prot ...

Cell Press - Elsevier2011

Phosphorylation of synucleins by members of the Polo-like kinase family

Hilal Lashuel, Harald Hirling, Abid Oueslati, Martial Mbefo Kamdem

Phosphorylation of alpha-synuclein (alpha-syn) at Ser-129 is a hallmark of Parkinson disease and related synucleinopathies. However, the identity of the natural kinases and phosphatases responsible for regulating alpha-syn phosphorylation remain unknown. H ...

American Society for Biochemistry and Molecular Biology2010

Overly long centrioles and defective cell division upon excess of the SAS-4-related protein CPAP

Pierre Gönczy

The centrosome is the principal microtubule organizing center (MTOC) of animal cells. Accurate centrosome duplication is fundamental for genome integrity and entails the formation of one procentriole next to each existing centriole, once per cell cycle. Th ...

2009

PLK-1 asymmetry contributes to asynchronous cell division of C. elegans embryos

Pierre Gönczy

Acquisition of lineage-specific cell cycle duration is an important feature of metazoan development. In Caenorhabditis elegans, differences in cell cycle duration are already apparent in two-cell stage embryos, when the larger anterior blastomere AB divide ...

2008

The tumor microenvironment and its contribution to tumor evolution toward metastasis

Cancer cells acquire cell-autonomous capacities to undergo limitless proliferation and survival through the activation of oncogenes and inactivation of tumor suppressor genes. Nevertheless, the formation of a clinically relevant tumor requires support from ...

Springer-Verlag2008

SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells

Pierre Gönczy

The mechanisms that ensure centrosome duplication are poorly understood. In Caenorhabditis elegans, ZYG-1, SAS-4, SAS-5 and SPD-2 are required for centriole formation. However, it is unclear whether these proteins have functional homologues in other organi ...

2005