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Concept

Transfert d'embryon

Publications associées (30)

Inheritance of H3K9 methylation regulates genome architecture in Drosophila early embryos

Fides Zenk

Constitutive heterochromatin is essential for transcriptional silencing and genome integrity. The establishment of constitutive heterochromatin in early embryos and its role in early fruitfly development are unknown. Lysine 9 trimethylation of histone H3 ( ...

Springernature2024

Sperm-contributed centrioles segregate stochastically into blastomeres of 4-cell stage Caenorhabditis elegans embryos

Pierre Gönczy, Fernando Romero Balestra

Whereas both sperm and egg contribute nuclear genetic material to the zygote in metazoan organisms, the inheritance of other cellular constituents is unequal between the 2 gametes. Thus, 2 copies of the centriole are contributed solely by the sperm to the ...

GENETICS SOCIETY AMERICA2023

The nuclear-to-cytoplasmic ratio drives cellularization in the close animal relative Sphaeroforma arctica

Omaya Pierre Dudin, Marine Olivetta

The ratio of nuclear content to cytoplasmic volume (N/C ratio) is a key regulator driving the maternal-to -zy-gotic transition in most animal embryos. Altering this ratio often impacts zygotic genome activation and de-regulates the timing and outcome of em ...

CELL PRESS2023

Microfluidic devices for automated analysis of ingested bacteria in the Caenorhabditis elegans intestine

Farzad Rezaeianaran

In this thesis, we explored on-chip high-resolution imaging of the fate of intestinal bacteria and bacterial products in Caenorhabditis elegans (C. elegans). In the first part, we carried out high-resolution z-stack fluorescent imaging of Red Fluorescent P ...

EPFL2023

How are cells instructed to form somite boundaries by the zebrafish segmentation clock?

Olivier François Venzin

In vertebrate embryos, the elongating body axis is patterned via the sequential and rhyth-mic production of segments from a posterior unsegmented tissue called the presomitic mesoderm (PSM). This process is controlled by a population of cellular oscillator ...

EPFL2023

Targeted search for scaling genes reveals matrix metalloproteinase 3 as a scaler of the dorsal-ventral pattern in Xenopus laevis embryos

Daria Korotkova

How embryos scale patterning according to size is still not fully understood. Through in silico screening and analysis of reaction-diffusion systems that could be responsible for scaling, we predicted the existence of genes whose expression is sensitive to ...

Cell Press - Elsevier2022

NMR spectroscopy of a single mammalian early stage embryo

Jürgen Brugger, Giovanni Boero, Marco Grisi, Gaurasundar Marc Conley, Kyle Joel Rodriguez

The resolving power, chemical sensitivity and non-invasive nature of NMR have made it an established technique for in vivo studies of large organisms both for research and clinical applications. NMR would clearly be beneficial for analysis of entities at t ...

Academic Press Inc - Elsevier Science2022

In vitro endoderm emergence and self-organisation in the absence of extraembryonic tissues and embryonic architecture

Stefano Davide Vianello

In humans, mice, and other mammals key internal organs such as the gut, the lungs, the pancreas, and the liver all derive from the same embryonic tissue: the endoderm. The development of all of these structures thus depends on a same set of early cells, an ...

EPFL2022

KRAB zinc finger protein-mediated control of transposon-embedded regulatory sequences from human germline to early embryos

Alexandra Iouranova

Transposable elements (TEs), also called jumping genes, are genetic elements capable of changing their position within the genome of their host. They make up large fractions of genomes, including 45% of human DNA content, according to current estimates. S ...

EPFL2021

Device and process for tissue-engineering and regenerative medicine

Thomas Michael Braschler, Olaia Maria Naveiras Torres-Quiroga, Josefine Catharina Pedersen Tratwal, Amélie Barbara Hildegarde Béduer, Martina Genta, Daniel Naveed Tavakol

A process and device for direct in-vitro to in-vivo transfer of a living microcarrier culture by controlled dehydration into a viscoelastic material capable of cell-protection during delivery. One particular purpose of the present invention is that of prov ...

2021

Automated phenotyping of Caenorhabditis elegans embryos with a high-throughput-screening microfluidic platform

Martinus Adela Maria Gijs, Matteo Cornaglia, Raphaël Etienne Jean Trouillon, Hüseyin Baris Atakan

The nematode Caenorhabditis elegans has been extensively used as a model multicellular organism to study the influence of osmotic stress conditions and the toxicity of chemical compounds on developmental and motility-associated phenotypes. However, the sev ...

2020

Blind killing of both male and female Drosophila embryos by a natural variant of the endosymbiotic bacterium Spiroplasma poulsonii

Bruno Lemaitre, Florent François Masson, Samuel Rommelaere, Juan Camilo Paredes Escobar, Mario Gonzalo Garcia Arraez

Spiroplasma poulsonii is a vertically transmitted endosymbiont of Drosophila melanogaster that causes male-killing, that is the death of infected male embryos during embryogenesis. Here, we report a natural variant of S. poulsonii that is efficiently verti ...

WILEY2020

Unequal cleavage of the C. elegans zygote ensures robust embryogenesis

Radek Jankele

Asymmetric cell divisions play key roles in generating cellular diversity during embryogenesis, and in tissue homeostasis during postembryonic life. Particular asymmetric cell divisions producing differentially sized daughter cells were described in many s ...

EPFL2020

Patterning and mechanics of somite boundaries in zebrafish embryos

Andrew Charles Oates, Sundar Ram Naganathan

The body axis of vertebrates is subdivided into repetitive compartments called somites, which give rise primarily to the segmented architecture of the musculoskeletal system in the adult body. Somites form in a sequential and rhythmic manner in embryos and ...

2020

Fractal Analysis And Related Forms Of Complexity Of Embryo Development. Is There A New Tool For Human Embryo Selection?

Maria-Alexandra Paun

A total number of 1092 embryos, originated from 168 couples that underwent IVF cycles, were analyzed in this study. The embryos were cultivated in a continuous culture and monitored using the time lapse system EmbryoScope. The microscopic images of the fer ...

UNIV POLITEHNICA BUCHAREST, SCI BULL2019

Understanding the Mechanobiology of Early Mammalian Development through Bioengineered Models

Matthias Lütolf, Stefano Davide Vianello

Research in developmental biology has been recently enriched by a multitude of in vitro models recapitulating key milestones of mammalian embryogenesis. These models obviate the challenge posed by the inaccessibility of implanted embryos, multiply experime ...

Cell Press - Elsevier2019

Automated Platform for Long-Term Culture and High-Content Phenotyping of Single C. elegans Worms

Martinus Adela Maria Gijs, Johan Auwerx, Matteo Cornaglia, Laurent Mouchiroud, Elena Katsyuba, Hüseyin Baris Atakan, Rongrong Xiang

The nematode Caenorhabditis elegans is a suitable model organism in drug screening. Traditionally worms are grown on agar plates, posing many challenges for long-term culture and phenotyping of animals under identical conditions. Microfluidics allows for ' ...

Nature Research2019

Functional analysis of RIP toxins from the Drosophila endosymbiont Spiroplasma poulsonii

Bruno Lemaitre, Florent François Masson, Mario Gonzalo Garcia Arraez, Juan Camilo Paredes Escobar

BackgroundInsects frequently live in close relationship with symbiotic bacteria that carry out beneficial functions for their host, like protection against parasites and viruses. However, in some cases, the mutualistic nature of such associations is put in ...

2019

DPPA2 and DPPA4 are necessary to establish a 2C‐like state in mouse embryonic stem cells

Didier Trono, Julien Léonard Duc, Alexandre Coudray, Alberto De Iaco

After fertilization of the transcriptionally silent oocyte, expression from both parental chromosomes is launched through zygotic genome activation (ZGA), occurring in the mouse at the 2-cell (2C) stage. Among the first elements to be transcribed are the D ...

2019

Long-term imaging, automated high-content phenotyping and sorting of C. elegans larvae and embryos using microfluidic platforms

Hüseyin Baris Atakan

C. elegans is frequently studied to address fundamental biological questions motivated by its short life cycle, small size, hermaphrodite behavior and well-annotated genome. In this work, we present novel microfluidic platforms that support long-term cultu ...

EPFL2019