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Concept

Hémoglobine

Publications associées (29)

Oxygen reduction catalyzed by a fluorinated tetraphenylporphyrin free base at liquid/liquid interfaces

Hubert Girault, Anne-Clémence Corminboeuf, Bin Su, Manuel Alejandro Méndez Agudelo, Imren Hatay

The diprotonated form of a fluorinated free base porphyrin, namely 5-(p-aminophenyl)-10,15,20-tris(pentafluorophenyl)porphyrin (H(2)FAP), can catalyze the reduction of oxygen by a weak electron donor, namely ferrocene (Fc). At a water/1,2-dichloroethane in ...

American Chemical Society2010

Probing the Electronic Structure of the Hemoglobin Active Center in Physiological Solutions

Majed Chergui

Soft-x-ray absorption spectroscopy at the L2,3 edge of the iron center in bovine Hb and hemin under physiol. conditions is reported for the first time. Spectra of the same compds. in solid form are presented for comparison. Striking differences in the elec ...

2009

Efficient oxygen transfer by surface aeration in shaken cylindrical containers for mammalian cell cultivation at volumetric scales up to 1,000 liters

Alfio Quarteroni, Florian Maria Wurm, David Hacker, Xiuwei Zhang, Marco Discacciati, Mathieu Benjamin Stettler, Nicola Parolini

Cylindrical containers agitated by orbital shaking are being developed as simple and cost-effective bioreactor systems for the cultivation of mammalian cells. Here the oxygen transfer capacities of containers with nominal volumes from 50 mL to 2000 L were ...

2009

Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Benjamin Rappaz

Red blood cell (RBC) parameters such as morphology, volume, refractive index, and hemoglobin content are of great importance for diagnostic purposes. Existing approaches require complicated calibration procedures and robust cell perturbation. As a result, ...

2008

Extraction of haemoglobin from human blood by affinity precipitation using a haptoglobin-based stimuli-responsive affinity macroligand

Gisela Stocker Majd

Affinity precipitation was compared to affinity chromatography and batch adsorption as the final purification step in a protocol for the isolation of haemoglobin from human blood. Haptoglobin was the affinity ligand. The first steps on the process were rea ...

2008

Autofluorescence detection of tumors in the human lung - spectroscopical measurements in situ, in an in vivo model and in vitro

Georges Wagnières, Tanja Gabrecht

To detect bronchial carcinoma by autofluorescence, we measured the spectra of tumor and normal tissue in situ, in an in vivo model and in vitro by fiber optic spectrometer and two-dimensional resolved microspectroscopy. The in situ measurements were perfor ...

Elsevier2008

The affinity precipitation for the isolation of biomolecules

Gisela Stocker Majd

Product isolation increasingly becomes a major bottleneck in molecular biotechnology. This is especially the case for the sensitive proteineous substances (recombinant proteins, antibodies) and lately also DNA, usually produced in low concentration. The cu ...

EPFL2007

Digital holographic microscopy applied to life sciences

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Benjamin Rappaz, Florian Charrière

Digital holographic microscopy (DHM) is applied to life sciences applications and demonstrate its capability of real-time imaging and quantitative measurements of physiological parameters such as cell volume or mean cell hemoglobin concentration (MCHC) of ...

IEEE2007

Sulfide-binding hemoglobins: effects of mutations on active-site flexibility

Majed Chergui

The dynamics of Hb I (HbI) from the clam Lucina pectinata, from wild-type sperm whale (SW) myoglobin, and from the L29F/H64Q/V68F triple mutant of SW, both unligated and bound to hydrogen sulfide (H2S), have been studied in mol. dynamics simulations. Featu ...

2006