Laboratory of Experimental Biophysics

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Single-molecule localization microscopy (SMLM) can reveal nanometric details of biological samples, but its high phototoxicity hampers long-term imaging in live specimens. A significant part of this phototoxicity stems from repeated irradiations that are n ...

The accurate identification of the three-dimensional quantitative shape of a cell nucleus is now possible without fluorescent staining by applying computational segmentation to refractive index tomograms recorded in the flow cytometry mode. ...

The topic of this thesis is the development of new algorithmic reconstruction methods for quantitative phase imaging (QPI). In the past decade, advanced QPI has emerged as a valuable tool to study label-free biological samples and uncover their 3D structur ...

Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited microscopy techniques and can image biological structures at the molecular scal ...

Imaging live cells in their native environment is crucial for the understanding of complex biological phenomena. Modern optical microscopy methods such as fluorescence super-resolution microscopy are increasingly combined with complementary, label-free tec ...

Rhodamines are the most important class of fluorophores for applications in live-cell fluorescence microscopy. This is mainly because rhodamines exist in a dynamic equilibrium between a fluorescent zwitterion and a nonfluorescent but cell-permeable spirocy ...

Defects in solid-state systems can be both detrimental, deteriorating the quality of materials, or desired, thanks to the novel functionality they bring. Optically active point defects, producing fluorescent light, are a great example of the latter. Natura ...

Single-molecule localization microscopy (SMLM) is a fluorescence microscopy technique that achieves super-resolution imaging by sequentially activating and localizing random sparse subsets of fluorophores. Each activated fluorophore emits light that then s ...

High-resolution live-cell imaging is necessary to study complex biological phenomena. Modern fluorescence microscopy methods are increasingly combined with complementary, label-free techniques to put the fluorescence information into the cellular context. ...

A fluorescence correlation contrast is a straightforward approach to super-resolution imaging. Combining a SPAD array with a novel detection scheme (ISM), we obtain images with up to x4 times resolution enhancement. ...

Localization microscopy is a super-resolution imaging technique that relies on the spatial and temporal separation of blinking fluorescent emitters. These blinking events can be individually localized with a precision significantly smaller than the classic ...

Single-molecule localization microscopy (SMLM) is a powerful method for the imaging of cellular structures. This modality delivers nanoscale resolution by sequentially activating a subset of fluorescent molecules and by extracting their super-resolved posi ...

Laboratory of Experimental Biophysics

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Long-Term, Single-Molecule Imaging of Proteins in Live Cells with Photoregulated Fluxional Fluorophores

Stain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry

Nonlinear Inverse Problems in Quantitative Phase Imaging

Single-molecule localization microscopy

Biophysical applications of correlative scanning probe and super-resolution microscopy

Systematic Tuning of Rhodamine Spirocyclization for Super-resolution Microscopy

Exploring optically active defects in wide-bandgap materials using fluorescence microscopy

Diffraction Tomography from Single-Molecule Localization Microscopy: Numerical Feasibility

Correlative 3D microscopy of single cells using super-resolution and scanning ion-conductance microscopy

SPAD array technology enables fluctuation-contrast super-resolution in a confocal microscope

Parameter-free rendering of single-molecule localization microscopy data for parameter-free resolution estimation

Optical diffraction tomography from single-molecule localization microscopy