Publication

Total synthesis of TASP 4a molecules by solid phase methods

1990
Conference Papers

Abstract

A report from a symposium on the recently developed strategy for protein de novo design, termed template-assembled synthetic proteins (TASPs), aimed to overcome the protein folding problem by constructing peptide sequences exhibiting nonlinear chain connectivities. In order to elucidate the potential of solid phase peptide synthesis for building up this new family of macromols., TASPs with the following structural features were designed: 1) a cyclic (1-10) template, Ac-Cys-Lys-Ala-Lys-Pro-Gly-Lys-Ala-Lys-Cys-NH2, and 2) four identical chains covalently bound to the e-amino function of the lysines. These chains were 11, 15, and 18 amino acids long as shown: Glu-Leu-Leu-|Glu-Ala-Leu-Glu-|Lys-Ala-Leu-Lys-Glu-Ala-Leu-Ala-Lys-Leu-Gly-. The peptides were assembled using a combination of tert-butoxycarbonyl (Boc) and 9-fluorenylmethoxycarbonyl (Fmoc) strategies on a methylbenzhydrylamine solid support. Conformational anal., using CD of the TASPs and individual chains, suggest a definite participation of the template in inducing and maintaining the predicted a-helical secondary structure of the 4-helix bundle. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/fffbe160-38f0-4f08-9fea-b38eb2051d59

About this result

This page is automatically generated and may contain information that is not correct, complete, up-to-date, or relevant to your search query. The same applies to every other page on this website. Please make sure to verify the information with EPFL's official sources.

Graph Chat

Chat with Graph Search

Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.

DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.

1990
Conference Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/fffbe160-38f0-4f08-9fea-b38eb2051d59

About this result

Related concepts (2)

Amino acid

Amino acids are organic compounds that contain both amino and carboxylic acid functional groups. Although over 500 amino acids exist in nature, by far the most important are the α-amino acids, from which proteins are composed. Only 22 α-amino acids appear in the genetic code of all life. Amino acids can be classified according to the locations of the core structural functional groups, as alpha- (α-), beta- (β-), gamma- (γ-) or delta- (δ-) amino acids; other categories relate to polarity, ionization, and side chain group type (aliphatic, acyclic, aromatic, containing hydroxyl or sulfur, etc.

Calcitonin

Calcitonin is a 32 amino acid peptide hormone secreted by parafollicular cells (also known as C cells) of the thyroid (or endostyle) in humans and other chordates in the ultimopharyngeal body. It acts to reduce blood calcium (Ca2+), opposing the effects of parathyroid hormone (PTH). Its importance in humans has not been as well established as its importance in other animals, as its function is usually not significant in the regulation of normal calcium homeostasis. It belongs to the calcitonin-like protein family.