Publication

Smart SU-8 Pillars Implemented in a Microfluidic Bioreactor for Continuous Measurement of Glucose

Nico de Rooij
2010
Conference Papers

Abstract

In this contribution we explore a new and simple approach for immobilizing enzymes like glucose oxidase on SU-8 surfaces to develop a smart substrate integrated in microfluidics. SU-8 is a well known photoresist often used in microfluidic prototyping. Immobilization of enzymes on such substance can open new possibilities in the microfabrication of enzyme biosensors and bioreactors. To demonstrate the consistency of this approach, we describe the design, fabrication and the simple functionalization of a microfluidic bioreactor employing smart SU-8 pillars for continuous amperometric measurement of glucose. The results reveal the possibility of simply binding enzymes on SU-8 surface. Moreover, a significant improvement in the linear response range is observed compared to the previous published amperometric microfluidic glucose sensors [1,2]. (C) 2010 Published by Elsevier Ltd.

Official source

https://infoscience.epfl.ch/entities/publication/fd82d9ac-0f1a-486d-9374-d2f1e38fe277

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Nico de Rooij
2010
Conference Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/fd82d9ac-0f1a-486d-9374-d2f1e38fe277

About this result

Related concepts (24)

Enzyme inhibitor

An enzyme inhibitor is a molecule that binds to an enzyme and blocks its activity. Enzymes are proteins that speed up chemical reactions necessary for life, in which substrate molecules are converted into products. An enzyme facilitates a specific chemical reaction by binding the substrate to its active site, a specialized area on the enzyme that accelerates the most difficult step of the reaction.

Enzyme

Enzymes (ˈɛnzaɪmz) are proteins that act as biological catalysts by accelerating chemical reactions. The molecules upon which enzymes may act are called substrates, and the enzyme converts the substrates into different molecules known as products. Almost all metabolic processes in the cell need enzyme catalysis in order to occur at rates fast enough to sustain life. Metabolic pathways depend upon enzymes to catalyze individual steps.

Glucose oxidase

The glucose oxidase enzyme (GOx or GOD) also known as notatin (EC number 1.1.3.4) is an oxidoreductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. This enzyme is produced by certain species of fungi and insects and displays antibacterial activity when oxygen and glucose are present. Glucose oxidase is widely used for the determination of free glucose in body fluids (medical testing), in vegetal raw material, and in the food industry.

Related publications (2)

Smart SU-8 Pillars Implemented in a Microfluidic Bioreactor for Continuous Measurement of Glucose

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Characterization of the erythrocyte response to glucose levels by impedance spectroscopy

Identification and control of oxidative metabolism in Saccharomyces cerevisiae during transient growth using calorimetric measurements