Publication

Microsecond melting and revitrification of cryo samples with a correlative light electron microscopy setup

Marcel Drabbels
2025

Abstract

An apparatus for observing dynamic systems by time-resolved microscopy has an optical microscope including a cryo stage for receiving a cryo sample and maintaining the cryo sample at a cryogenic temperature, a heating system configured to temporarily heat at least a portion of the cryo sample such that the portion of the cryo sample melts to a liquid state, temporarily resides in the liquid state, and then revitrifies, and an image detector associated with the optical microscope for detecting magnified images of the cryo sample generated by the optical microscope. Detected images may be recorded and analyzed to inform on-the-fly adjustment of heating power level delivered to the cryo sample. In a correlative light-electron microscopy method, the revitrified cryo sample is observed using an electron microscope.

Official source

https://infoscience.epfl.ch/entities/publication/fd13696b-09b9-40b6-a206-74b695347be3

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Marcel Drabbels
2025

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/fd13696b-09b9-40b6-a206-74b695347be3

About this result

Related concepts (32)

Cryogenic electron microscopy

Cryogenic electron microscopy (cryo-EM) is a cryomicroscopy technique applied on samples cooled to cryogenic temperatures. For biological specimens, the structure is preserved by embedding in an environment of vitreous ice. An aqueous sample solution is applied to a grid-mesh and plunge-frozen in liquid ethane or a mixture of liquid ethane and propane. While development of the technique began in the 1970s, recent advances in detector technology and software algorithms have allowed for the determination of biomolecular structures at near-atomic resolution.

Electron microscope

An electron microscope is a microscope that uses a beam of electrons as a source of illumination. They use electron optics that are analogous to the glass lenses of an optical light microscope. As the wavelength of an electron can be up to 100,000 times shorter than that of visible light, electron microscopes have a higher resolution of about 0.1 nm, which compares to about 200 nm for light microscopes.

Optical microscope

The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century. Basic optical microscopes can be very simple, although many complex designs aim to improve resolution and sample contrast. The object is placed on a stage and may be directly viewed through one or two eyepieces on the microscope.

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Microsecond melting and revitrification of cryo samples with a correlative light electron microscopy setup

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