In situ product removal (ISPR) in whole cell biotechnology during the last twenty years

This review sums up the activity in the field of in situ product removal in whole cell bioprocesses over the last 20 yr. It gives a complete summary of ISPR operations with microbial cells and cites a series of interesting ISPR applications in plant and animal cell technol. All the ISPR projects with microbial cells are categorized according to their products, their ISPR techniques, and their applied configurations of the ISPR set-up. Research on ISPR application has primarily increased in the field of microbial prodn. of aromas and org. acids such lactic acid over the last ten years. Apart from the field of de novo formation of bioproducts, ISPR is increasingly applied to microbial bioconversion processes. However, despite of the large no. of microbial whole cell ISPR projects (approx. 250), very few processes have been transferred to an industrial scale. The proposed processes have mostly been too complex and consequently not cost effective. Therefore, this review emphasizes that the planning of a successful whole cell ISPR process should not only consider the choice of ISPR technique according to the physicochem. properties of the product, but also the potential configuration of the whole process set-up. Furthermore, addnl. process aspects, biol. and legal constraint need to be considered from the very beginning for the design of an ISPR project. Finally, future trends of new, modified or improved ISPR techniques are given. [on SciFinder (R)]