Publication

Controlled Immobilization of Membrane Proteins to Surfaces for Fourier Transform Infrared Investigations

Horst Arthur Vogel, Wolf-Peter Ulrich, Per Rigler
2004
Journal Articles

Abstract

We show that it is possible to immobilize membrane proteins uniformly and reversibly as self-assembled (sub)monolayers on nitrilotriacetic acid-covered sensor surfaces via hexahistidine sequences present either in the protein or in lipid membranes. Fourier transform IR spectra of such self-assembled (sub)monolayers deliver important structural information of the membrane proteins and are suited to screen the function of cellular receptors. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/f3858f0e-1ce5-4a49-aefe-a2b4ec88b398

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Horst Arthur Vogel, Wolf-Peter Ulrich, Per Rigler
2004
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/f3858f0e-1ce5-4a49-aefe-a2b4ec88b398

About this result

Related concepts (29)

Lipid bilayer

The lipid bilayer (or phospholipid bilayer) is a thin polar membrane made of two layers of lipid molecules. These membranes are flat sheets that form a continuous barrier around all cells. The cell membranes of almost all organisms and many viruses are made of a lipid bilayer, as are the nuclear membrane surrounding the cell nucleus, and membranes of the membrane-bound organelles in the cell. The lipid bilayer is the barrier that keeps ions, proteins and other molecules where they are needed and prevents them from diffusing into areas where they should not be.

Cell membrane

The cell membrane (also known as the plasma membrane or cytoplasmic membrane, and historically referred to as the plasmalemma) is a biological membrane that separates and protects the interior of a cell from the outside environment (the extracellular space). The cell membrane consists of a lipid bilayer, made up of two layers of phospholipids with cholesterols (a lipid component) interspersed between them, maintaining appropriate membrane fluidity at various temperatures.

Membrane protein

Membrane proteins are common proteins that are part of, or interact with, biological membranes. Membrane proteins fall into several broad categories depending on their location. Integral membrane proteins are a permanent part of a cell membrane and can either penetrate the membrane (transmembrane) or associate with one or the other side of a membrane (integral monotopic). Peripheral membrane proteins are transiently associated with the cell membrane.

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Controlled Immobilization of Membrane Proteins to Surfaces for Fourier Transform Infrared Investigations

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