Publication

Online simultaneous monitoring of ammonia and glutamine in a hollow-fiber reactor using flow injection analysis

Urs von Stockar, Ian William Marison
1996
Journal Articles

Abstract

A FIA method has been developed and fully characterized for the simultaneous detection of ammonia and glutamine in culture media. Ammonia detection is based upon a chem. method and does not require an electrode. This species diffuses across a hydrophobic porous membrane into an indicator soln., the absorbance of which is continuously monitored using a LED and a phototransistor. Glutamine was detd. by a difference method in which ammonia was measured before and after passage through a module contg. immobilized glutaminase. Very good linearity was obsd. in the range 0-4 mM for both species. Total anal. time was 22 min. This FIA method was used to accurately monitor ammonia and glutamine on line for over 600 h for a hybridoma culture performed in a hollow-fiber reactor. FIA measurements were in good agreement with off-line measurements. No instrument failures occurred, thanks to systematic cleaning and maintenance procedures. This FIA method is a very attractive tool for the monitoring, and possibly control, of continuous cultures over extended periods. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/ee001c60-ff5b-41cd-9b73-6c4df166bd99

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Urs von Stockar, Ian William Marison
1996
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/ee001c60-ff5b-41cd-9b73-6c4df166bd99

About this result

Related concepts (32)

Finite difference method

In numerical analysis, finite-difference methods (FDM) are a class of numerical techniques for solving differential equations by approximating derivatives with finite differences. Both the spatial domain and time interval (if applicable) are discretized, or broken into a finite number of steps, and the value of the solution at these discrete points is approximated by solving algebraic equations containing finite differences and values from nearby points.

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Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. This technique is also called micropropagation. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. They need to be kept at body temperature (37 °C) in an incubator.

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In mathematics and computational science, the Euler method (also called the forward Euler method) is a first-order numerical procedure for solving ordinary differential equations (ODEs) with a given initial value. It is the most basic explicit method for numerical integration of ordinary differential equations and is the simplest Runge–Kutta method. The Euler method is named after Leonhard Euler, who first proposed it in his book Institutionum calculi integralis (published 1768–1870).

Related publications (12)

Online simultaneous monitoring of ammonia and glutamine in a hollow-fiber reactor using flow injection analysis

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