Extended depth-of-focus by digital holographic microscopy

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Structured light generation having broad applications in different optical fields, is the topic of this thesis. Our structured light generation strategy is based on applying periodic microoptical elements at the refraction-diffraction limit, under a focuse ...

Imaging live cells in their native environment is crucial for the understanding of complex biological phenomena. Modern optical microscopy methods such as fluorescence super-resolution microscopy are increasingly combined with complementary, label-free tec ...

A fluorescence correlation contrast is a straightforward approach to super-resolution imaging. Combining a SPAD array with a novel detection scheme (ISM), we obtain images with up to x4 times resolution enhancement. ...

A modern day light microscope has evolved from a tool devoted to making primarily empirical observations to what is now a sophisticated , quantitative device that is an integral part of both physical and life science research. Nowadays, microscopes are fou ...

High-resolution live-cell imaging is necessary to study complex biological phenomena. Modern fluorescence microscopy methods are increasingly combined with complementary, label-free techniques to put the fluorescence information into the cellular context. ...

Rhodamines are the most important class of fluorophores for applications in live-cell fluorescence microscopy. This is mainly because rhodamines exist in a dynamic equilibrium between a fluorescent zwitterion and a nonfluorescent but cell-permeable spirocy ...

Single-molecule localization microscopy (SMLM) is a powerful method for the imaging of cellular structures. This modality delivers nanoscale resolution by sequentially activating a subset of fluorescent molecules and by extracting their super-resolved posi ...

Mycobacteria have unique cell envelopes, surface properties and growth dynamics, which all play a part in the ability of these important pathogens to infect, evade host immunity, disseminate and to resist antibiotic challenges. Recent atomic force microsco ...

The organisation of molecules into dynamic cells, and collaboration of many of those cells over a billion years led to the evolution of human life. During the last century, biologists then began to unravel the marvels of cellular organisation with ever inc ...

This thesis presents a combined experimental and theoretical study of the classical and quantum magnetization dynamics in single magnetic adatoms and molecules, and on the classical and quantum coherent control thereof. First, a detailed description of t ...

Refractive microlenses are nowadays widely used in optical systems. Characterizing their surface is essential to ensure their quality and to optimize their fabrication process. This is realized by optical surface profilers thanks to their vertical resoluti ...

Optical microscopy is one widely used tool to study cell functions and the interaction of molecules at a sub-cellular level. Optical microscopy techniques can be broadly divided into two categories: partially coherent and incoherent. Coherent microscopy te ...

Image relighting is attracting increasing interest due to its various applications. From a research perspective, image relighting can be exploited to conduct both image normalization for domain adaptation, and also for data augmentation. It also has multip ...

Extended depth-of-focus by digital holographic microscopy

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Structured light generation by refractive-diffractive micro optical array illuminators

Biophysical applications of correlative scanning probe and super-resolution microscopy

SPAD array technology enables fluctuation-contrast super-resolution in a confocal microscope

QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy

Correlative 3D microscopy of single cells using super-resolution and scanning ion-conductance microscopy

Systematic Tuning of Rhodamine Spirocyclization for Super-resolution Microscopy

Optical diffraction tomography from single-molecule localization microscopy

Seeing and Touching the Mycomembrane at the Nanoscale

Organisation and dynamics of mitochondria and their RNA

Quantum dynamics in individual surface spins

Correction of spherical surface measurements by confocal microscopy

Development of novel experimental and computational methods for three-dimensional coherent and super-resolution microscopy

NTIRE 2021 Depth Guided Image Relighting Challenge