Publication

Point spread function model for microscopic image deconvolution in digital holographic microscopy

Christian Depeursinge, Etienne Cuche
2003
Conference Papers

Abstract

A simple model is proposed in order to evaluate the complex amplitude point spread function (intensity and phase) of a microscope objective. The model is based on the Fresnel diffraction theory and takes also into account the possible optical aberrations. Experimental evaluation of this amplitude point spread function has been carried out by using a holographic set-up and 66 nanometers gold spheres as punctual objects. The measured values are then compared with the theoretical predicted model.

Official source

https://infoscience.epfl.ch/entities/publication/eaa8a344-b64e-4d45-872a-5f559e9cf266

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Christian Depeursinge, Etienne Cuche
2003
Conference Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/eaa8a344-b64e-4d45-872a-5f559e9cf266

About this result

Related concepts (32)

Optical microscope

The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century. Basic optical microscopes can be very simple, although many complex designs aim to improve resolution and sample contrast. The object is placed on a stage and may be directly viewed through one or two eyepieces on the microscope.

Microscopy

Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy. Optical microscopy and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron beams interacting with the specimen, and the collection of the scattered radiation or another signal in order to create an image.

Point spread function

The point spread function (PSF) describes the response of a focused optical imaging system to a point source or point object. A more general term for the PSF is the system's impulse response; the PSF is the impulse response or impulse response function (IRF) of a focused optical imaging system. The PSF in many contexts can be thought of as the extended blob in an image that represents a single point object, that is considered as a spatial impulse. In functional terms, it is the spatial domain version (i.e.

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Point spread function model for microscopic image deconvolution in digital holographic microscopy

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