Publication

Chinese Hamster Ovary Cells, Recombinant Protein Production

Florian Maria Wurm
2010

Abstract

Recombinant therapeutic proteins represent a major class of biopharmaceuticals. They are produced in various hosts including bacteria, yeast, cultivated mammalian and plant cells, and transgenic plants and animals. Chinese hamster ovary (CHO) cells are the most widely used mammalian host for their production. Currently, about one-third of all approved recombinant therapeutic proteins are produced in this cell line. This article is a summary of the molecular and cellular processes involved in therapeutic protein production with an emphasis on the generation and cultivation of recombinant CHO cell lines.

Official source

https://infoscience.epfl.ch/entities/publication/e6606dec-3489-48a8-be71-0156b9084082

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Florian Maria Wurm
2010

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/e6606dec-3489-48a8-be71-0156b9084082

About this result

Related concepts (32)

Chinese hamster ovary cell

Chinese hamster ovary (CHO) cells are an epithelial cell line derived from the ovary of the Chinese hamster, often used in biological and medical research and commercially in the production of recombinant therapeutic proteins. They have found wide use in studies of genetics, toxicity screening, nutrition and gene expression, particularly to express recombinant proteins. CHO cells are the most commonly used mammalian hosts for industrial production of recombinant protein therapeutics.

Protein production

Protein production is the biotechnological process of generating a specific protein. It is typically achieved by the manipulation of gene expression in an organism such that it expresses large amounts of a recombinant gene. This includes the transcription of the recombinant DNA to messenger RNA (mRNA), the translation of mRNA into polypeptide chains, which are ultimately folded into functional proteins and may be targeted to specific subcellular or extracellular locations.

Cell culture

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. This technique is also called micropropagation. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. They need to be kept at body temperature (37 °C) in an incubator.

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Chinese Hamster Ovary Cells, Recombinant Protein Production

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