Publication

Selective immobilization of active 6-O-Alkylguanine DNA alkyltransferase for its determination by transferring a coupling group to the enzyme from a 6-O-Alkylguanine derivative

Kai Johnsson
2000
Patents

Abstract

The invention concerns the introduction of a coupling group to 6-O-Alkylguanine DNA alkyltransferase from a 6-O-Alkylguanine-coupling group deriv. that enables the immobilization of the enzyme for subsequent anal. The coupling group is bound covalently to the enzyme; the immobilization to the surface is either by covalent or non-covalent binding. The coupling group can be biotin; the 6-O-Alkylguanine DNA alkyltransferase-biotin is immobilized to streptavidin coated surface. Independently from the coupling group, the enzyme can be fluorescent or enzyme-labeled for anal. purposes. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/e4bca914-7d52-4289-9cfa-ca99392c919d

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Kai Johnsson
2000
Patents

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/e4bca914-7d52-4289-9cfa-ca99392c919d

About this result

Related concepts (13)

Non-covalent interaction

In chemistry, a non-covalent interaction differs from a covalent bond in that it does not involve the sharing of electrons, but rather involves more dispersed variations of electromagnetic interactions between molecules or within a molecule. The chemical energy released in the formation of non-covalent interactions is typically on the order of 1–5 kcal/mol (1000–5000 calories per 6.02 molecules). Non-covalent interactions can be classified into different categories, such as electrostatic, π-effects, van der Waals forces, and hydrophobic effects.

Ligand (biochemistry)

In biochemistry and pharmacology, a ligand is a substance that forms a complex with a biomolecule to serve a biological purpose. The etymology stems from Latin ligare, which means 'to bind'. In protein-ligand binding, the ligand is usually a molecule which produces a signal by binding to a site on a target protein. The binding typically results in a change of conformational isomerism (conformation) of the target protein. In DNA-ligand binding studies, the ligand can be a small molecule, ion, or protein which binds to the DNA double helix.

Coupling reaction

In organic chemistry, a coupling reaction is a type of reaction in which two reactant molecules are bonded together. Such reactions often require the aid of a metal catalyst. In one important reaction type, a main group organometallic compound of the type R-M (where R = organic group, M = main group centre metal atom) reacts with an organic halide of the type R'-X with formation of a new carbon-carbon bond in the product R-R'. The most common type of coupling reaction is the cross coupling reaction. Richard F.

Related publications (2)

Selective immobilization of active 6-O-Alkylguanine DNA alkyltransferase for its determination by transferring a coupling group to the enzyme from a 6-O-Alkylguanine derivative

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Heterogeneous coupling by virtual control methods

Highly cis-selective Wittig reactions employing a-heterosubstituted ylides