The effect of the functional interaction between PGC-1α and Parkin on mitochondrial quality control in Parkinson's disease

Although several genetic factors have been directly associated to Parkinsonâ s disease (PD), no single pathway has emerged to explain the etiology of this neurodegenerative disease. To further understand the cause of Parkinsonâ s disease (PD), it is therefore important to deter-mine if and which functional interactions exist between gene products linked to this complex brain disorder. Mutations in the gene encoding the E3 ubiquitin ligase Parkin are associated to autosomal recessive PD with early onset. Although the Parkin protein carries out various critical func-tions in the cell, its role in mitochondria quality control, by inducing the autophagic degrada-tion of damaged mitochondria, has attracted a lot of attention. Furthermore, Parkin has been found to control the transcription of PGC-1α, a master regulator of mitochondrial biogenesis. Based on the important role that mitochondria are considered to play in PD, and the evi-dence for the coordinated expression of Parkin and PGC-1α, we sought to explore in neurons how these two factors functionally interact to regulate mitochondria turnover and quality control. In cortical neuronal cultures, we found that Parkin and PGC-1α have synergistic effects on mitochondrial biogenesis, and that the co-expression of these two factors leads to a dramatic increase in the number of mitochondria per cell. We further explored the effects of these fac-tors on the mitochondrial function. Parkin was shown to enhance the maximal respiration rate of neurons exposed to a mitochondrial uncoupling agent only in the presence of PGC-1α. Consistent with this effect, the co-expression of Parkin and PGC-1α leads to rapid recovery of the mitochondrial membrane potential following mitochondrial uncoupling. Next, we explored the molecular changes, which may underlie the cooperation between Par-kin and PGC-1α. The expression of Tfam, an important mitochondrial transcription factor, was found to be controlled synergistically by these two factors. Furthermore, PGC-1α en-hances the turnover of Mfn2, a key ubiquitylation target of Parkin. This result suggests that part of the functional interaction between PGC-1α and Parkin may be mediated via the co-regulation of Mfn2, a critical factor in mitochondrial physiology which controls the dynamics of mitochondria as well as their association with the endoplasmic reticulum (ER). In the rat substantia nigra, Parkin activity has significant protective effects on the survival and function of nigral dopaminergic neurons in which the chronic expression of PGC-1α is in-duced. Ultrastructural analysis shows that the conjunction of these two factors controls the density of mitochondria and their interaction with the ER. In PGC-1α-expressing dopamin-ergic neurons, the co-expression of mutated variants of Parkin associated with familial PD leads to accelerated degeneration. Overall, this study shows that Parkin, in conjunction with the transcription co-regulator PGC-1α, may play an