Publication

Improved growth and methane production conditions for Methanobacterium thermoautotrophicum

Urs von Stockar, Ian William Marison
1993
Journal Articles

Abstract

M. thermoautotrophicum was grown on a defined mineral salts medium under strictly anaerobic conditions with H2 and CO2 as the sole energy and C sources, resp. The cultivation medium was optimized with respect to non-org. components, including Se(IV), W(VI), N, Ni(II), Fe(II), Co(II), and Mo(VI). S2- concn. in the medium was maintained const. using an online regulatory system by the addn. of 0.5M Na2S. A max. supply rate of 0.6 vvm of a mixt. of 80% H2 and 20% CO2 was achieved for the gaseous substrates. Under these conditions, a sp. growth rate of 0.30/h and a cell concn. of 4.8 g cell dry wt. (DW)/L, representing a 140% increase over previously published results, were obtained. The growth yield of 2.3 g DW/mol CH4 was similar to published values. However, the overall sp. productivity was enhanced from 11 to 24 mmol CH4/g DW-h, corresponding to an improvement of 120%. [on SciFinder (R)]

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https://infoscience.epfl.ch/entities/publication/caddab06-0a83-432f-9e0c-5485ca309a7c

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Urs von Stockar, Ian William Marison
1993
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/caddab06-0a83-432f-9e0c-5485ca309a7c

About this result

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