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Determination of the levels of heavy metal ions would support assessment of sources and pathways of water pollution. However, traditional spatial assessment by manual sampling and off-site detection in the laboratory is expensive and time-consuming and requires trained personnel. Aiming to fill the gap between on-site automatic approaches and laboratory techniques, we developed an autonomous sensing boat for on site heavy metal detection using square-wave anodic stripping voltammetry. A fluidic sensing system was developed to integrate into the boat as the critical sensing component and could detect