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The present invention relates to a method for testing a substance interacting with a target molecule in a cell by fluorescence correlation spectroscopy (FCS) by adding a test substance to the cell and determining a distribution of diffusion coefficients for the fluorescently labelled target molecule. Preferably, the target molecule is a nuclear hormone receptor, e.g. the estrogen receptor.
Christoph Merten, Jatin Panwar
Edoardo Charbon, Claudio Bruschini