Publication

Analysis of cellular structure and dynamics with digital holographic microscopy

Christian Depeursinge, Florian Charrière
2007
Conference Papers

Abstract

Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically, the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We discuss some approaches allowing to directly obtain separate measurements of the thickness and the refractive index (RI) of a given living cell from the phase signal. © 2007 SPIE-OSA.

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https://infoscience.epfl.ch/entities/publication/c12ceacb-eee7-45c6-a6b4-480a8d8e1eea

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Christian Depeursinge, Florian Charrière
2007
Conference Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/c12ceacb-eee7-45c6-a6b4-480a8d8e1eea

About this result

Related concepts (32)

Quantitative phase-contrast microscopy

FORCETOC Quantitative phase contrast microscopy or quantitative phase imaging are the collective names for a group of microscopy methods that quantify the phase shift that occurs when light waves pass through a more optically dense object. Translucent objects, like a living human cell, absorb and scatter small amounts of light. This makes translucent objects much easier to observe in ordinary light microscopes. Such objects do, however, induce a phase shift that can be observed using a phase contrast microscope.

Phase-contrast microscopy

NOTOC Phase-contrast microscopy (PCM) is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. Phase shifts themselves are invisible, but become visible when shown as brightness variations. When light waves travel through a medium other than a vacuum, interaction with the medium causes the wave amplitude and phase to change in a manner dependent on properties of the medium.

Holography

Holography is a technique that enables a wavefront to be recorded and later re-constructed. Holography is best known as a method of generating real , but it also has a wide range of other applications. In principle, it is possible to make a hologram for any type of wave. A hologram is made by superimposing a second wavefront (normally called the reference beam) on the wavefront of interest, thereby generating an interference pattern which is recorded on a physical medium.

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Analysis of cellular structure and dynamics with digital holographic microscopy

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