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Probing Cysteine Reactivity in Proteins by Mass Spectrometric EC-Tagging

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High Resolution Mass Spectrometry for Studying the Interactions of Cisplatin with Oligonucleotides

Paul Joseph Dyson, Yury Tsybin, Christian Hartinger, Hisham Ben Hamidane

Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) has been used to probe the interaction of the anticancer drug cisplatin with oligonucleotides. The binding kinetics, the nature of the adducts formed, and the location of the binding s ...

2008

Extended Bottom-Up Proteomics with Secreted Aspartic Protease Sap9

Yury Tsybin, Kristina Srzentic, Unige Anna Laskay

We investigate the benefits and experimental feasibility of approaches enabling the shift from short (1.7 kDa on average) peptides in bottom-up proteomics to about twice longer (similar to 3.2 kDa on average) peptides in the so-called extended bottom-up pr ...

Elsevier Science Bv2014

Spectroscopic studies of peptide fragments produced by collision-induced dissociation

Oleg Aseev

Tandem mass spectrometry (MS/MS) combined with collision-induced dissociation (CID) is a key method for primary structure determination of peptides and proteins. Collisionally activated peptides undergo statistical dissociation, forming a series of backbon ...

EPFL2014

Thiol-targeted microspray mass spectrometry of peptides and proteins through on-line EC-tagging

Loïc Dayon

Modification strategies targeting specific amino acids in proteins are widespread in proteomic analysis. Cysteine residues have received deep consideration in view of their nucleophilic properties and their occurrence in the proteome. A recently developed ...

EPFL2006

Multitrack electrospray chips

Hubert Girault, Niels Erik Lion, Michel Prudent, Loïc Dayon, Mélanie Abonnenc

Multitrack electrospray chips (MTEC) were fabricated by UV-photoablation of polyethylene terephthalate (PET) substrates. They are composed of an array of up to six microchannels that are successively used as electrospray ionization (ESI) emitters for mass ...

Wiley-Blackwell2006

Protein-nanoparticle interactions: the effects of surface compositional and structural heterogeneity are scale dependent

Francesco Stellacci, Randy Patrick Carney

Nanoparticles (NPs) in the biological environment are exposed to a large variety and concentration of proteins. Proteins are known to adsorb in a 'corona' like structure on the surface of NPs. In this study, we focus on the effects of surface compositional ...

Royal Society of Chemistry2013

Microfluidic front-end technologies for protein electrospray mass spectrometry

Niels Erik Lion

In the present work, a polymer microfabricated microsprayer is characterised and tested for the mass spectrometric analysis of biomolecules, in particular in the context of protein mass spectrometry and proteomics. As the core of this work deals with analy ...

EPFL2006

Development of New Bicyclic Peptide Formats and their Application in Directed Evolution

Shiyu Chen

Cyclic peptides have a range of properties that make them attractive as therapeutics such as high binding affinity, good target selectivity and low toxicity. While most peptides in the clinic are derived from nature, various powerful in vitro evolution tec ...

EPFL2013

On-Line Counting of Cysteine Residues in Peptides during Electrospray Ionization by Electrogenerated Tags and Their Application to Protein Identification

Hubert Girault, Christophe Roussel, Niels Erik Lion, Michel Prudent, Loïc Dayon

The electrochemically induced mass spectrometric tagging of cysteines by substituted hydroquinones was studied for peptides in a classical electrospray solvent (i.e., MeOH/H2O/AcOH 50/49/1). The tagging efficiency was tested with different hydroquinone com ...

2005

Electron Transfer Dissociation Mass Spectrometry of Hemoglobin on Clinical Samples

Yury Tsybin

A mass spectrometry-based assay combining the specificity of selected reaction monitoring and the protein ion activation capabilities of electron transfer dissociation was developed and employed for the rapid identification of hemoglobin variants from whol ...

Springer2012

The Quantum Magnetism of Individual Manganese-12-Acetate Molecular Magnets Anchored at Surfaces

Klaus Kern, Stephan Rauschenbach, Markus Ternes, Gordon Rinke

The high intrinsic spin and long spin relaxation time of manganese-12-acetate (Mn-12) makes it an archetypical single molecular magnet. While these characteristics have been measured on bulk samples, questions remain whether the magnetic properties replica ...

American Chemical Society (ACS)2012

Electrochemical Multi-Tagging of Cysteinyl Peptides during Microspray Mass Spectrometry: Numerical Simulation of Consecutive Reactions in a Microchannel

Hubert Girault, Loïc Dayon

On-line electrogeneration of mass tags in a microspray emitter is used to quantify the number of cysteine groups in a given peptide. A finite-element simulation of the multi-step process yields the relative distribution and concentration of tags, untagged ...

2005

UV radiation induces genome-mediated, site-specific cleavage in viral protein

Tamar Kohn, Yury Tsybin, Laure Menin, Krista Rule Wigginton, Michele Cascella, Anne Thérèse Sigstam, Hisham Ben Hamidane

Much research has been dedicated to understanding the molecular basis of UV damage to biomolecules, yet many questions remain regarding the specific pathways involved. Here we describe a genome-mediated mechanism that causes sitespecific virus protein clea ...

2012

Fragmentation methods on the balance: unambiguous top–down mass spectrometric characterization of oxaliplatin–ubiquitin binding sites

Paul Joseph Dyson, Yury Tsybin, Christian Hartinger

The interaction between oxaliplatin and the model protein ubiquitin (Ub) was investigated in a top-down approach by means of high-resolution electrospray ionization mass spectrometry (ESI-MS) using diverse tandem mass spectrometric (MS/MS) techniques, incl ...

Springer-Verlag2012

Reaction of C60 with Hydrogen Gas: In Situ Monitoring and Pathways

Yury Tsybin

Kinetics and pathways of C60 reaction with hydrogen gas were studied in a broad temperature interval and over extended periods of time. Specifically, hydrogenation was monitored in situ at high temperature and high hydrogen pressure conditions using the gr ...

American Chemical Society2011