Publication

Investigating cellular signalling reactions by micro- and nanotechnology

Horst Arthur Vogel
2004
Conference Papers

Abstract

Classically, cellular signalling is investigated measuring different optical or elec. properties of either single cells or microliter vols. of suspensions of live cells. Here we show how ligand binding to cell surface receptors and subsequent signalling reactions can be monitored in single submicrometer sized vesicles derived from biol. cells. These vesicles are the smallest autonomous containers capable of performing cellular signaling reactions, thus opening the door to downscale anal. of cellular functions to the micro-/nm and femto-/aL range. We describe a method that allows the massively parallel isolation of attoliter exptl. vols. and their self-assembled positioning with 100-nm precision in ordered arrays on surfaces. This miniaturization opens novel routes in functional proteomics such as multiplexing single cell bioanalytics or investigating receptor mediated signalling in multiarray format. Single mol. spectroscopies of suitably labeled proteins are used to investigate ionotropic receptor and GPCR mediated signalling in this miniaturized format. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/beb7ae3f-4cd5-4320-82ef-206b0bd53c71

About this result

This page is automatically generated and may contain information that is not correct, complete, up-to-date, or relevant to your search query. The same applies to every other page on this website. Please make sure to verify the information with EPFL's official sources.

Graph Chat

Chat with Graph Search

Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.

DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.

Horst Arthur Vogel
2004
Conference Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/beb7ae3f-4cd5-4320-82ef-206b0bd53c71

About this result

Related concepts (32)

Cell signaling

In biology, cell signaling (cell signalling in British English) or cell communication is the ability of a cell to receive, process, and transmit signals with its environment and with itself. Cell signaling is a fundamental property of all cellular life in prokaryotes and eukaryotes. Signals that originate from outside a cell (or extracellular signals) can be physical agents like mechanical pressure, voltage, temperature, light, or chemical signals (e.g., small molecules, peptides, or gas).

G protein-coupled receptor

G protein-coupled receptors (GPCRs), also known as seven-(pass)-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptors, and G protein-linked receptors (GPLR), form a large group of evolutionarily related proteins that are cell surface receptors that detect molecules outside the cell and activate cellular responses. They are coupled with G proteins.

Cellular automaton

A cellular automaton (pl. cellular automata, abbrev. CA) is a discrete model of computation studied in automata theory. Cellular automata are also called cellular spaces, tessellation automata, homogeneous structures, cellular structures, tessellation structures, and iterative arrays. Cellular automata have found application in various areas, including physics, theoretical biology and microstructure modeling. A cellular automaton consists of a regular grid of cells, each in one of a finite number of states, such as on and off (in contrast to a coupled map lattice).

Related publications (15)

Investigating cellular signalling reactions by micro- and nanotechnology

Chat with Graph Search

Cellular pathways of carbon and nitrogen assimilation in kleptoplastic foraminiferal species

Visual backward masking deficits in schizophrenic patients are associated with polymorphisms in the nicotinic receptor alpha 7 subunit gene (CHRNA7)

Development of a microfluidic on-chip reverse transcription module for the analysis of hematopoietic single stem cells