Publication

On-line determination of animal cell concentration

Urs von Stockar, Ian William Marison, Martin Rhiel, Patrick Pugeaud
2001
Journal Articles

Abstract

A new approach for the indirect detn. of cell concn. in the case of nonconstant metabolic rates has been developed. The specific glucose-uptake rate was shown to be nonconstant in batch cultures of free suspended and immobilized CHO SSF3 cells. Time-independent models correlating the specific rate to the limiting substrate concn. were established, thus providing a continuous detn. of the specific rate through online measurement of the limiting substrate. The method could be applied to det. online cell concn. in both free suspended and immobilized cell cultures. Results were verified off-line by crystal violet nuclei counting. The predicted cell concn. was in very good agreement with the off-line ref. during the whole exponential-growth phase, until the specific glucose-uptake rate tended to zero. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/bd4f06fc-c3d0-4e8f-8ed0-5d1cc981da56

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Urs von Stockar, Ian William Marison, Martin Rhiel, Patrick Pugeaud
2001
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/bd4f06fc-c3d0-4e8f-8ed0-5d1cc981da56

About this result

Related concepts (30)

Blood sugar level

The blood sugar level, blood sugar concentration, blood glucose level, or glycemia, is the measure of glucose concentrated in the blood. The body tightly regulates blood glucose levels as a part of metabolic homeostasis. For a 70 kg (154 lb) human, approximately four grams of dissolved glucose (also called "blood glucose") is maintained in the blood plasma at all times.

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Glucose is a sugar with the molecular formula . Glucose is overall the most abundant monosaccharide, a subcategory of carbohydrates. Glucose is mainly made by plants and most algae during photosynthesis from water and carbon dioxide, using energy from sunlight, where it is used to make cellulose in cell walls, the most abundant carbohydrate in the world. In energy metabolism, glucose is the most important source of energy in all organisms. Glucose for metabolism is stored as a polymer, in plants mainly as starch and amylopectin, and in animals as glycogen.

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Related publications (4)

On-line determination of animal cell concentration

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