Publication

Principles of an efficient new method for the removal of ammonia from animal cell cultures using hydrophobic membranes

Urs von Stockar, Ian William Marison
1994
Journal Articles

Abstract

To overcome ammonia inhibition in animal cell culture, a method utilizing porous polytetrafluoroethylene membranes with an assocd. pH gradient was investigated. Model expts. without cells were carried out in three different reactor configurations suitable for animal cell culture processes to characterize mass transfer properties of the process. The actual fluxes of ammonia across the membrane are of the same order of magnitude as the typical ammonia prodn. rate in an animal cell process, thus indicating the potential of the method for preventing the accumulation of toxic ammonia in cell culture. [on SciFinder (R)]

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https://infoscience.epfl.ch/entities/publication/ba6851ef-98d3-4a4d-afb3-a0646ae5647b

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Urs von Stockar, Ian William Marison
1994
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/ba6851ef-98d3-4a4d-afb3-a0646ae5647b

About this result

Related concepts (32)

Cell culture

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. This technique is also called micropropagation. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. They need to be kept at body temperature (37 °C) in an incubator.

Tissue culture

Tissue culture is the growth of tissues or cells in an artificial medium separate from the parent organism. This technique is also called micropropagation. This is typically facilitated via use of a liquid, semi-solid, or solid growth medium, such as broth or agar. Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows.

Cell cycle

The cell cycle, or cell-division cycle, is the series of events that take place in a cell that causes it to divide into two daughter cells. These events include the duplication of its DNA (DNA replication) and some of its organelles, and subsequently the partitioning of its cytoplasm, chromosomes and other components into two daughter cells in a process called cell division. In cells with nuclei (eukaryotes, i.e., animal, plant, fungal, and protist cells), the cell cycle is divided into two main stages: interphase and the mitotic (M) phase (including mitosis and cytokinesis).

Related publications (13)

Principles of an efficient new method for the removal of ammonia from animal cell cultures using hydrophobic membranes

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