Publication

The effect of cell line, transfection procedure and reactor conditions on the glycosylation of recombinant human anti-rhesus D IGG1

Urs von Stockar, Ian William Marison
1999
Conference Papers

Abstract

An anti-Rhesus D antibody was produced in various cell-lines and cell-types which were either stable or transient transfected and grown under varying culture conditions. Glycosylation anal. of the IgG1 was carried out mainly by HPAEC methods. The antibody was N-glycosylated only at the conserved glycosylation site at Asn-297 carrying complex type N-glycans as usually present on IgG. The core structure was entirely fucosylated and did not carry intersecting G1cNAc for all antibodies analyzed. Prodn. in SP2/0, CHO and HEK-293 cells resulted in significantly different oligosaccharide patterns. The influence of the cultivation conditions on the glycosylation was usually less than the influence of cell-type. The glycosylation pattern of IgG produced in transient transfected CHO cells was different than in stable transfected CHO cells but the differences obsd. might be also due to different culture conditions. Further factors influencing the glycosylation were medium compn. and the type of bioreactor used for the cultivation. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/9eb16ff9-0cb1-4af2-965b-20a0740b47a1

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Urs von Stockar, Ian William Marison
1999
Conference Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/9eb16ff9-0cb1-4af2-965b-20a0740b47a1

About this result

Related concepts (32)

Cell culture

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. This technique is also called micropropagation. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. They need to be kept at body temperature (37 °C) in an incubator.

Immortalised cell line

An immortalised cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division. The cells can therefore be grown for prolonged periods in vitro. The mutations required for immortality can occur naturally or be intentionally induced for experimental purposes. Immortal cell lines are a very important tool for research into the biochemistry and cell biology of multicellular organisms.

Stem cell

In multicellular organisms, stem cells are undifferentiated or partially differentiated cells that can differentiate into various types of cells and proliferate indefinitely to produce more of the same stem cell. They are the earliest type of cell in a cell lineage. They are found in both embryonic and adult organisms, but they have slightly different properties in each. They are usually distinguished from progenitor cells, which cannot divide indefinitely, and precursor or blast cells, which are usually committed to differentiating into one cell type.

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