Publication

Design of Oligonucleotide Arrays at Interfaces

Horst Arthur Vogel
1999
Journal Articles

Abstract

The surface attachment and detection of DNA probes are essential in the design of nucleic acid-based biosensors. A new strategy for the covalent immobilization of single-stranded oligonucleotides on gold-covered planar supports is presented. Optimization of the surface d. in the resulting DNA arrays permits a high hybridization efficiency to be achieved. Surface plasmon resonance and, for the first time, ATR-FTIR spectroscopy are used to follow in situ the oligonucleotide layer formation and the subsequent complementary strand hybridization. Such well-defined, covalently immobilized oligonucleotide arrays can find application in the development of novel DNA-based sensors for mutation detection and gene mapping as well as in studies of nucleic acid-ligand interactions. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/9e0cdff0-0bd0-4936-872a-a22f90089919

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Horst Arthur Vogel
1999
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/9e0cdff0-0bd0-4936-872a-a22f90089919

About this result

Related concepts (32)

DNA microarray

A DNA microarray (also commonly known as DNA chip or biochip) is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome. Each DNA spot contains picomoles (10−12 moles) of a specific DNA sequence, known as probes (or reporters or oligos). These can be a short section of a gene or other DNA element that are used to hybridize a cDNA or cRNA (also called anti-sense RNA) sample (called target) under high-stringency conditions.

DNA sequencing

DNA sequencing is the process of determining the nucleic acid sequence – the order of nucleotides in DNA. It includes any method or technology that is used to determine the order of the four bases: adenine, guanine, cytosine, and thymine. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery. Knowledge of DNA sequences has become indispensable for basic biological research, DNA Genographic Projects and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics.

DNA

Deoxyribonucleic acid (diːˈɒksᵻˌraɪboʊnjuːˌkliːᵻk,_-ˌkleɪ-; DNA) is a polymer composed of two polynucleotide chains that coil around each other to form a double helix. The polymer carries genetic instructions for the development, functioning, growth and reproduction of all known organisms and many viruses. DNA and ribonucleic acid (RNA) are nucleic acids. Alongside proteins, lipids and complex carbohydrates (polysaccharides), nucleic acids are one of the four major types of macromolecules that are essential for all known forms of life.

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Design of Oligonucleotide Arrays at Interfaces

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