Publication

Decellularization of bovine articular cartilage by supercritical carbon dioxide as a matrix for cartilage repair

2017
Student Projects

Abstract

Decellularized extracellular matrix (ECM) derived scaﬀolds serve as potential candidates in the application of cartilage regeneration to guide cells into proliferating and maintaining the structural zones of articular cartilage. ECM tissue matrices oﬀer framework and structural support and guid-ance in a tissue engineering cartilage scaﬀold. To generate such a scaﬀold, decellularization must be performed in a fast and eﬃcient manner to remove cellular products to minimally damage and alterate the ECM. Here, we introduce a novel method used in cartilage decellularization involving a standard cartilage pre-treatment process paired with the surfactant, LS-54, and supercritical carbon dioxide to quickly remove cellular components, with DNA removal reduced by 80%, while sterilizing the matrix. Large pores and no visual nuclei were seen in the tissue. Recellularization was studied with mechanical stimulation using compression release induced suction (CRIS) and an in-house perfusion setup.

Official source

https://infoscience.epfl.ch/entities/publication/9d70268e-2f13-4155-9224-d4a784e6867a

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2017
Student Projects

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/9d70268e-2f13-4155-9224-d4a784e6867a

About this result

Related concepts (32)

Tissue engineering

Tissue engineering is a biomedical engineering discipline that uses a combination of cells, engineering, materials methods, and suitable biochemical and physicochemical factors to restore, maintain, improve, or replace different types of biological tissues. Tissue engineering often involves the use of cells placed on tissue scaffolds in the formation of new viable tissue for a medical purpose but is not limited to applications involving cells and tissue scaffolds.

Cartilage

Cartilage is a resilient and smooth type of connective tissue. In tetrapods, it covers and protects the ends of long bones at the joints as articular cartilage, and is a structural component of many body parts including the rib cage, the neck and the bronchial tubes, and the intervertebral discs. In other taxa, such as chondrichthyans, but also in cyclostomes, it may constitute a much greater proportion of the skeleton. It is not as hard and rigid as bone, but it is much stiffer and much less flexible than muscle.

Hyaline cartilage

Hyaline cartilage is the glass-like (hyaline) and translucent cartilage found on many joint surfaces. It is also most commonly found in the ribs, nose, larynx, and trachea. Hyaline cartilage is pearl-gray in color, with a firm consistency and has a considerable amount of collagen. It contains no nerves or blood vessels, and its structure is relatively simple. Hyaline cartilage is covered externally by a fibrous membrane known as the perichondrium or, when it's along articulating surfaces, the synovial membrane.

Related publications (2)

Decellularization of bovine articular cartilage by supercritical carbon dioxide as a matrix for cartilage repair

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