Publication

Short synthesis of labeled and unlabeled 6Z,9Z,12Z,15-hexadecatetraenoic acid as metabolic probes for biosynthetic studies on diatoms

2004
Journal Articles

Abstract

Max-Planck-Institute for Chemical Ecology, Hans-Knoll-Str. 8, D-07745 Jena, Germany. pohnert@ice.mpg.de We describe a short synthesis of the unusual polyunsaturated 6Z,9Z,12Z,15-hexadecatetraenoic acid found in marine and fresh water diatoms. Using a one pot reductive bis-Wittig-olefination, the homoconjugated tetraene backbone of the fatty acid can be generated from easy available precursors. Reductive olefination allows the non-statistical dissymmetrisation of a symmetrical bis-Wittig salt as key synthon. This short sequence was also applied to the generation of the corresponding 9,10-[(2)H(2)] labeled fatty acid. If administered to cell fragments of Thalassiosira rotula 9,10-[(2)H(2)]-6Z,9Z,12Z,15-hexadecatetraenoic acid is transformed oxidatively to the aldehyde 1,2-[(2)H(2)]-2E,4E/Z,7-octatrienal which is involved in the chemical defense of this alga. Using the synthetic standard it could be shown that the C16:4 omega1 fatty acid is released upon wounding of T. rotula cells. The synthesis with the labeled bis-Wittig salt is of general use and can also be applied for the fast generation of other internally labeled functionalized and non-functionalized polyunsaturated fatty acids. To our knowledge this represents the first synthesis of 6Z,9Z,12Z,15-hexadecatetraenoic acid.

Official source

https://infoscience.epfl.ch/entities/publication/992541de-cf42-4fbb-8dbe-ec4d252c1d68

About this result

This page is automatically generated and may contain information that is not correct, complete, up-to-date, or relevant to your search query. The same applies to every other page on this website. Please make sure to verify the information with EPFL's official sources.

Graph Chat

Chat with Graph Search

Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.

DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.

2004
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/992541de-cf42-4fbb-8dbe-ec4d252c1d68

About this result

Related concepts (32)

Fatty acid

In chemistry, particularly in biochemistry, a fatty acid is a carboxylic acid with an aliphatic chain, which is either saturated or unsaturated. Most naturally occurring fatty acids have an unbranched chain of an even number of carbon atoms, from 4 to 28. Fatty acids are a major component of the lipids (up to 70% by weight) in some species such as microalgae but in some other organisms are not found in their standalone form, but instead exist as three main classes of esters: triglycerides, phospholipids, and cholesteryl esters.

Omega-3 fatty acid

Omega−3 fatty acids, also called Omega−3 oils, ω−3 fatty acids or n−3 fatty acids, are polyunsaturated fatty acids (PUFAs) characterized by the presence of a double bond, three atoms away from the terminal methyl group in their chemical structure. They are widely distributed in nature, being important constituents of animal lipid metabolism, and they play an important role in the human diet and in human physiology. The three types of omega−3 fatty acids involved in human physiology are α-linolenic acid (ALA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA).

Essential fatty acid

Essential fatty acids, or EFAs, are fatty acids that humans and other animals must ingest because the body requires them for good health, but cannot synthesize them. Only two fatty acids are known to be essential for humans: alpha-linolenic acid (an omega-3 fatty acid) and linoleic acid (an omega-6 fatty acid). These are supplied to the body either as the free fatty acid, or more commonly as some glyceride derivative. Deficiency in these fatty acids is rare.