Publications related to Quantitative phase-digital holographic microscopy: a new imaging modality to identify original cellular biomarkers of diseases

Related publications (37)

Structural investigations of brain and skin tissues in Parkinson's and related diseases, using correlative light and electron microscopy

Parkinson's disease (PD), Dementia with Lewy Bodies (DLB) and Multiple System Atrophy (MSA) are neurodegenerative disorders collectively termed synucleinopathies, as they all present proteinaceous pathological aggregates of alpha-synuclein (aSyn) in the br ...

EPFL2025

Bioengineering of morphologically and functionally accurate in vitro intestinal models

Jakob Josef Langer

Organoids are stem cell-derived 3D mimetics of tissues and organs, representing an invaluable tool for modelling tissue homeostasis and disease biology, holding great potential for drug development and clinical applications. However, organoid formation rel ...

EPFL2025

Optimizing Ionic Conductivity of Lithium in Li7PS6 Argyrodite via Dopant Engineering

Nicola Marzari, Sokseiha Muy

Li-containing argyrodites represent a promising family of Li-ion conductors, with several derived compounds exhibiting room-temperature ionic conductivity >1 mS/cm, making them attractive as potential candidates for electrolytes in solid-state Li-ion batte ...

AMER CHEMICAL SOC2025

Live-cell imaging powered by computation

Suliana Manley

The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. The prevailing challenge in live-cell fluorescence microscopy is capturing intra-cellular dynamics while preservi ...

Nature Portfolio2024

3D printed large amplitude torsional microactuators powered by ultrasound

Mahmut Selman Sakar, Amit Yedidia Dolev, Mehdi Ali Gadiri, Junsun Hwang

Here, we introduce a design, fabrication, and control methodology for large amplitude torsional microactuators powered by ultrasound. The microactuators are 3D printed from two polymers with drastically different elastic moduli as a monolithic compliant me ...

2024

Deciphering the nature of cell state transitions in single cells using quantitative modeling of temporal dynamics

Alex Russell Lederer

Cells are the smallest operational units of living systems. Through synthesis of various biomolecules and exchange of signals with the environment, cells tightly regulate their composition to realize a specific functional state. The transformation of a cel ...

EPFL2024

Tangentially viewing phase contrast imaging for turbulence measurements in toroidal fusion devices

Stefano Coda

Phase contrast imaging (PCI) is an established and powerful technique for measuring density fluctuations in plasmas and has been successfully applied to several fusion devices. Rooted in a concept first developed for microscopy, PCI belongs to the category ...

2024

Bioconcentration Assessment of Three Cationic Surfactants in Permanent Fish Cell Lines

Kristin Schirmer

Cationic surfactants are used in many industrial processes and in consumer products with concurrent release into the aquatic environment, where they may accumulate in aquatic organisms to regulatoryly relevant thresholds. Here, we aimed to better understan ...

2024

Structure of tetrameric forms of the serotonin-gated 5-HT3A receptor ion channel

Horst Arthur Vogel, Menno Berend Tol

Multimeric membrane proteins are produced in the endoplasmic reticulum and transported to their target membranes which, for ion channels, is typically the plasma membrane. Despite the availability of many fully assembled channel structures, our understandi ...

SPRINGERNATURE2024

Ghost imaging using two SPAD array detectors: a parameter study towards the realization of a 3D quantum microscope

Edoardo Charbon, Claudio Bruschini, Paul Mos, Yang Lin

Quantum ghost imaging can be an important tool in making optical measurements. One of the most useful aspects of ghost imaging is the unique ability to correlate two sets of independently collected information. We aim to use the principles of ghost imaging ...

Spie-Int Soc Optical Engineering2024

Compact and effective photon-resolved image scanning microscope

Giorgio Tortarolo

Fluorescence confocal laser-scanning microscopy (LSM) is one of the most popular tools for life science research. This popularity is expected to grow thanks to single-photon array detectors tailored for LSM. These detectors offer unique single-photon spati ...

Spie-Soc Photo-Optical Instrumentation Engineers2024

A synthetic phase-contrast imaging diagnostic with spatial filtering for gyrokinetic simulations

Stefano Coda, Stephan Brunner, Gabriele Merlo, Justin Richard Ball, Aylwin Iantchenko

A Phase-contrast imaging (PCI) diagnostic provides measurements of line-integrated electron density fluctuations. Localisation along the laser beam path can be achieved with a spatial filter that selects the wave-vector directions of the fluctuations contr ...

2023

Practical computational imaging by use of spatiotemporal light modulation: from simulations to applications in biological microscopy

François Thierry M Marelli

Optical microscopy is an essential tool for biologists, who are often faced with the need to overcome the spatial and temporal resolution limitations of their devices to capture finer details. As upgrading imaging hardware is expensive, computational metho ...

EPFL2023

Automated Dual-Mode Cell Monitoring To Simultaneously Explore Calcium Dynamics and Contraction-Relaxation Kinetics within Drug-Treated Stem Cell-Derived Cardiomyocytes

Pierre Marquet, Gerardo Turcatti, Benjamin Rappaz, Bokyoung Kim

This manuscript proposes a new dual-mode cell imaging system for studying the relationships between calcium dynamics and the contractility process of cardiomyocytes derived from human-induced pluripotent stem cells. Practically, this dual-mode cell imaging ...

AMER CHEMICAL SOC2023

Time-Resolved Scanning Ion Conductance Microscopy and Single-Molecule Spectroscopy

Samuel Mendes Leitão

The evolution of the 3D morphology is at the center of many relevant biological processes ranging from cellular differentiation to cancer invasion and metastasis. Microscopy techniques, such as electron microscopy (EM), super-resolution (SR) optical micros ...

EPFL2023

Self-supervised Dense Representation Learning for Live-Cell Microscopy with Time Arrow Prediction

Martin Weigert, Benjamin Tobias Gallusser

State-of-the-art object detection and segmentation methods for microscopy images rely on supervised machine learning, which requires laborious manual annotation of training data. Here we present a self-supervised method based on time arrow prediction pre-t ...

Springer International Publishing Ag2023

Exploring Novel Modalities for Optical Diffraction Tomography

Amirhossein Saba Shirvan

In the past decade, optical diffraction tomography has gained a lot of attention for its ability to create label-free three-dimensional (3D) images of the refractive index distribution of biological samples using scattered fields measured through holograph ...

EPFL2023

An optimal preconditioned FFT-accelerated finite element solver for homogenization

Till Junge, Ali Falsafi

We generalize and provide a linear algebra-based perspective on a finite element (FE) ho-mogenization scheme, pioneered by Schneider et al. (2017)[1] and Leuschner and Fritzen (2018)[2]. The efficiency of the scheme is based on a preconditioned, well-scale ...

ELSEVIER SCIENCE INC2023

Stain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry

Demetri Psaltis, Joowon Lim

The accurate identification of the three-dimensional quantitative shape of a cell nucleus is now possible without fluorescent staining by applying computational segmentation to refractive index tomograms recorded in the flow cytometry mode. ...

Nature Publishing Group2022

Label-Free Long-Term Methods for Live Cell Imaging of Neurons: New Opportunities

Georg Fantner, Samuel Mendes Leitão, Zahra Ayar Dulabi

Time-lapse light microscopy combined with in vitro neuronal cultures has provided a significant contribution to the field of Developmental Neuroscience. The establishment of the neuronal polarity, i.e., formation of axons and dendrites, key structures resp ...

MDPI2023