Publication

A Physically Plausible Model for Rendering Highly Scattering Fluorescent Participating Media

2017
Working Papers

Abstract

We present a novel extension of the path tracing algorithm that is capable of treating highly scattering participating media in the presence of fluorescent structures. The extension is based on the formulation of the full radiative transfer equation when solved on a per-wavelength-basis, resulting in accurate model and unbiased algorithm for rendering highly scattering fluorescent participating media. The model accounts for the intrinsic properties of fluorescent dyes including their absorption and emission spectra, molar absorptivity and quantum yield and also their concentration. Our algorithm is applied to render highly scattering isotropic fluorescent solutions under different illumination conditions. The spectral performance of the model is validated against emission spectra of different fluorescent dyes that are of significance in spectroscopy. 18 pages, 7 figures, conference

Official source

https://infoscience.epfl.ch/entities/publication/978fee78-b24d-407f-a7e6-68350221c672

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2017
Working Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/978fee78-b24d-407f-a7e6-68350221c672

About this result

Related concepts (32)

Fluorescent tag

In molecular biology and biotechnology, a fluorescent tag, also known as a fluorescent label or fluorescent probe, is a molecule that is attached chemically to aid in the detection of a biomolecule such as a protein, antibody, or amino acid. Generally, fluorescent tagging, or labeling, uses a reactive derivative of a fluorescent molecule known as a fluorophore. The fluorophore selectively binds to a specific region or functional group on the target molecule and can be attached chemically or biologically.

Fluorophore

A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with several π bonds. Fluorophores are sometimes used alone, as a tracer in fluids, as a dye for staining of certain structures, as a substrate of enzymes, or as a probe or indicator (when its fluorescence is affected by environmental aspects such as polarity or ions).

Fluorescence

Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore a lower photon energy, than the absorbed radiation. A perceptible example of fluorescence occurs when the absorbed radiation is in the ultraviolet region of the electromagnetic spectrum (invisible to the human eye), while the emitted light is in the visible region; this gives the fluorescent substance a distinct color that can only be seen when the substance has been exposed to UV light.

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