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High-resolution cell outline segmentation and tracking from phase-contrast microscopy images

Related publications (31)

Time-Resolved Scanning Ion Conductance Microscopy and Single-Molecule Spectroscopy

The evolution of the 3D morphology is at the center of many relevant biological processes ranging from cellular differentiation to cancer invasion and metastasis. Microscopy techniques, such as electron microscopy (EM), super-resolution (SR) optical micros ...

EPFL2023

Microscopic Digital Image Correlation Analysis of Strain Distributions in Stainless Steel 316L Induced by Multiaxial Cyclic Loading

Elif Cansu Kursun

Fatigue damage in materials results in localized strain at the microstructural level. In many engineering components of the cooling circuits of nuclear power plants, where austenitic steels are used, the material experiences multiaxial cyclic loading, eith ...

EPFL2023

Mitochondrial nanomotion measured by optical microscopy

Sandor Kasas, María Inés Villalba, Priyanka Parmar

Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and de ...

Frontiers Media SA2023

Image scanning microscopy with a doughnut beam: signal strength and integrated intensity

Giorgio Tortarolo

We discuss the effects of image scanning microscopy using doughnut beam illumination on the properties of signal strength and integrated intensity. Doughnut beam illumination can give better optical sectioning and background rejection than Airy disk illumi ...

Optica Publishing Group2023

Background Rejection in Two-Photon Fluorescence Image Scanning Microscopy

Giorgio Tortarolo

We discuss the properties of signal strength and integrated intensity in two-photon excitation confocal microscopy and image scanning microscopy. The resolution, optical sectioning and background rejection are all improved over nonconfocal two-photon micro ...

MDPI2023

A synthetic phase-contrast imaging diagnostic with spatial filtering for gyrokinetic simulations

Stephan Brunner, Stefano Coda, Gabriele Merlo, Aylwin Iantchenko, Justin Richard Ball

A Phase-contrast imaging (PCI) diagnostic provides measurements of line-integrated electron density fluctuations. Localisation along the laser beam path can be achieved with a spatial filter that selects the wave-vector directions of the fluctuations contr ...

2023

Simple optical nanomotion method for single-bacterium viability and antibiotic response testing

Sandor Kasas, María Inés Villalba, Allan Bonvallat, Eugenia Rossetti

Antibiotic resistance is nowadays a major public health issue. Rapid antimicrobial susceptibility tests (AST) are one of the options to fight this deadly threat. Performing AST with single-cell sensitivity that is rapid, cheap, and widely accessible, is ch ...

National Academy of Sciences2023

Signal strength and integrated intensity in confocal and image scanning microscopy

Giorgio Tortarolo

The properties of signal strength and integrated intensity in a scanned imaging system are reviewed. These prop-erties are especially applied to confocal imaging systems, including image scanning microscopy. The integrated intensity, equal to the image of ...

Optica Publishing Group2023

Exploring Novel Modalities for Optical Diffraction Tomography

Amirhossein Saba Shirvan

In the past decade, optical diffraction tomography has gained a lot of attention for its ability to create label-free three-dimensional (3D) images of the refractive index distribution of biological samples using scattered fields measured through holograph ...

EPFL2023

Towards near-atomic resolution imaging of microsecond nanoscale dynamics

Gabriele Bongiovanni

Time-resolved electron microscopy has made significant progress in recent years, with some groups now working on instruments that offer attosecond temporal resolution. While much of the research in the field revolves around the improvement of temporal reso ...

EPFL2023

SAGTTA: SALIENCY GUIDED TEST TIME AUGMENTATION FOR MEDICAL IMAGE SEGMENTATION ACROSS VENDOR DOMAIN SHIFT

Seyedbehzad Bozorgtabar, Devavrat Tomar

Test time augmentation has been shown to be an effective approach to combat domain shifts in deep learning. Despite their promising performance levels, the interpretability of the underlying used models is however low. Saliency maps have been widely used i ...

IEEE2023